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Team:Hawaii/Notebook/2008-07-24
From 2008.igem.org
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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verification of parts=== :<strong> Krystle</strong> :* Colony PCR of nir+rbs, gfp+tt, gfpfusion+tt transformants :* Ra...) |
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Latest revision as of 05:26, 25 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of parts
- Krystle
- Colony PCR of nir+rbs, gfp+tt, gfpfusion+tt transformants
- Ran 3% gel for colony PCR reactions and plasmid preps of nir, slr1, slr2, pilA, GFP fusion, pRL1383a, B0015, and J04430.
Plasmid prep
- Krystle
- Inoculated 7 ml LB+amp with transformants for plasmid prep of nir+rbs, gfp+tt, gfpfusion+tt tomorrow
Drylab Work
Sequencing
- Grace
- Aligned and checked sequences received from CORE Hawaii for DNA sent in last week
Added BioBrick parts to registry
- Grace
- Added BB-pRL1383a, nir, pilA, slr2016, and GFP fusion parts to BioBrick registry
Discussion
- Sequencing for J33207 returned the sequence for E0026 (not sequenced). Need to run all plasmid preps of these two BioBricks on a gel to verify plasmid preps are properly labeled.
Quote of the Day
