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Team:Hawaii/Notebook/2008-07- 7
From 2008.igem.org
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===PCR of pRL1383a parts=== | ===PCR of pRL1383a parts=== | ||
:<strong> Margaret</strong> | :<strong> Margaret</strong> | ||
| + | [[Image:rep_mob_red_green.jpg|right|thumb|300px|Rep and Mob regions can be amplified by red/green taq.]] | ||
:* Amplification of ''rep'' and ''mob'' regions. Neither of these parts came out very well, so trying them with red and green taq. | :* Amplification of ''rep'' and ''mob'' regions. Neither of these parts came out very well, so trying them with red and green taq. | ||
Revision as of 03:06, 15 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Biobrick Extraction
- Margaret
- Extracted Biobricks BBa_E0240, BBa_I4032, BBa_pSB3K3
- Transformed into E. coli
- 7/08/08: checked and there are no transformants (I made a mistake about the death protein...), decided to drop this experiment until DB3.1 cells come in.
Oligonucleotide experiment
- Grace
- Ligated annealed products using 10-2 dilution of products
- Reran on EtBr stained 4% agarose gel (result: light bands for annealed products, correct size; no bands for ligated product)
- Ran annealed products (10-1 dilution) on SYBR Safe stained 2% agarose gel
- Restriction digested ligation from 7/3/08 using XbaI and PstI in NEBuffer 3
- Reran on EtBR stained 4% agarose gel
Plasmid prep
- Krystle
- Grew up E. coli from cryostock and performed an alkaline lysis plasmid prep for C0012
PCR of pRL1383a parts
- Margaret
- Amplification of rep and mob regions. Neither of these parts came out very well, so trying them with red and green taq.
Discussion
Quote of the Day
"I assume if there are jellyfish, it's not completely pelleted?" - KS
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
