Team:Hawaii/Notebook/2008-07- 7

From 2008.igem.org

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Things we did today

Wetlab work

Biobrick Extraction

Margaret
  • Extracted Biobricks BBa_E0240, BBa_I4032, BBa_pSB3K3
  • Transformed into E. coli
  • 7/08/08: checked and there are no transformants (I made a mistake about the death protein...), decided to drop this experiment until DB3.1 cells come in.

Oligonucleotide experiment

Grace
  • Ligated annealed products using 10-2 dilution of products
  • Reran on EtBr stained 4% agarose gel (result: light bands for annealed products, correct size; no bands for ligated product)
  • Ran annealed products (10-1 dilution) on SYBR Safe stained 2% agarose gel
  • Restriction digested ligation from 7/3/08 using XbaI and PstI in NEBuffer 3
  • Reran on EtBR stained 4% agarose gel

Plasmid prep

Krystle
  • Grew up E. coli from cryostock and performed an alkaline lysis plasmid prep for C0012

PCR of pRL1383a parts

Rep and Mob regions can be amplified by red/green taq.
Margaret
  • Amplification of rep and mob regions. Neither of these parts came out very well, so trying them with red and green taq.

Discussion

Quote of the Day

"I assume if there are jellyfish, it's not completely pelleted?" - KS


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