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Things we did today

Wetlab work

Test of lab's RE (cont.)

Grace

EtBr stained 1.2% agarose gel ran at 72V for 1.5 hours. Thirty microliters of RE digest reaction were loaded into each well.

• Ran RE digests from yesterday on a 1.2% agarose gel

Verified transformants

Grace

EtBr stained 2% agarose gel ran at 95V for 1 hour. Five microliters of PCR reaction were loaded into each well.

• Colony PCR of transformants to check for correct insert

• Ligated slr, pilA with GFPf (ES digested) instead of GFPf (XP digested). Still got colonies. Hm...

3A assembly

Grace

• Ligated p+r with g or s onto pSB1A2

• pnir+rbs with GFP, slr1, pilA
• plac+rbs with GFP, slr, pilA

• Transformed into DH5α

Drylab Work

Name of Task

name of person/people who performed the task

• Summary of task and what was done. Link to experiment for detailed notes if necessary.
• e.g. read through papers, worked on proposal, etc.

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

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