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Team:Hawaii/Notebook/2008-08-17
From 2008.igem.org
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! Blue colonies | ! Blue colonies | ||
|- | |- | ||
| - | | align=center|BB-pRL1383a + J33207 | + | | align=center|BB-pRL1383a + J33207 |
| align=center|LB + sp<sub>100</sub> + sm<sub>50</sub> + IPTG (1mM) + X-gal (2mM) | | align=center|LB + sp<sub>100</sub> + sm<sub>50</sub> + IPTG (1mM) + X-gal (2mM) | ||
|align=center| | |align=center| | ||
|align=center| | |align=center| | ||
|- | |- | ||
| - | | align=center|BB-pRL1383a + J33207 | + | | align=center|BB-pRL1383a + J33207 |
|align=center|LB + sp<sub>100</sub> + X-gal (2mM) | |align=center|LB + sp<sub>100</sub> + X-gal (2mM) | ||
|align=center| | |align=center| | ||
Revision as of 01:59, 17 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of transformants
- Grace
| DNA | Plate | Total colony forming units | Blue colonies |
|---|---|---|---|
| BB-pRL1383a + J33207 | LB + sp100 + sm50 + IPTG (1mM) + X-gal (2mM) | ||
| BB-pRL1383a + J33207 | LB + sp100 + X-gal (2mM) | ||
| BB-pRL1383a (negative control) | LB + sp100 + sm50 + IPTG (1mM) + X-gal (2mM) | ||
| no DNA (negative control) | LB + sp100 + sm50 + IPTG (1mM) + X-gal (2mM) |
- Colony PCR to verify insert
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
