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Things we did today

Wetlab work

Grace

EtBr stained 2% agarose gel ran at 95V for 1 hour. Five microliters of PCR reaction were loaded into each well.

DNA	Plate	Total colony forming units	Blue colonies
BB-pRL1383a + J33207	LB + sp₁₀₀ + sm₅₀ + IPTG (1mM) + X-gal (2mM)
BB-pRL1383a + J33207	LB + sp₁₀₀ + X-gal (2mM)
BB-pRL1383a (negative control)	LB + sp₁₀₀ + sm₅₀ + IPTG (1mM) + X-gal (2mM)
no DNA (negative control)	LB + sp₁₀₀ + sm₅₀ + IPTG (1mM) + X-gal (2mM)

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

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