Team:Hawaii/Notebook/2008-08-17
From 2008.igem.org
Projects | Events | Resources | ||
---|---|---|---|---|
Sponsors | Experiments | Milestones | Protocols | |
Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of transformants
- Grace
DNA | Plate | Total colony forming units | Blue colonies |
---|---|---|---|
BB-pRL1383a + J33207 | LB + sp100 + sm50 + IPTG (1mM) + X-gal (2mM) | 105 | 0 |
BB-pRL1383a + J33207 | LB + sp100 + X-gal (2mM) | 44 | 0 |
BB-pRL1383a (negative control) | LB + sp100 + sm50 + IPTG (1mM) + X-gal (2mM) | 44 | 0 |
no DNA (negative control) | LB + sp100 + sm50 + IPTG (1mM) + X-gal (2mM) | 0 | 0 |
- Colony PCR to verify insert
- ~325bp band is due to pRL1383a plasmid
- BB-pRL1383a + J33207 bands are smaller than (-) control bands, potential success in replacing GFP w/ lac device
- (-) bands due to contamination (no template added to PCR rxn) and GFP (religated plasmid, one RE did not cut)
- Restreaked colonies to purify
- Grew up colonies on TB+sp100 for plasmid prep
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]