Team:Hawaii/Notebook/2008-08-23

From 2008.igem.org

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:* PCR of insert of nir+rbs, nir (control), rbs+GFP, GFP (control)
===3A assembly===
===3A assembly===

Latest revision as of 01:48, 24 August 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Verification of transformants (cont.)

Grace
  • Plasmid prep of nir+rbs, rbs+GFP
Plasmid prep DNA concentration 260/280 260/230
nir+rbs 394.63 ng/μl 2.04 2.36
rbs+GFP 94.9 ng/μl 2.06 2.17
  • PCR of insert of nir+rbs, nir (control), rbs+GFP, GFP (control)

3A assembly

Grace
File:082308REdigests.jpg
EtBr stained 2% agarose gel ran at 60V for 3 hours. Thirty microliters of RE digest reaction were loaded into each well.
  • Gel purified RE digests
  • nir band did not show up on gel
  • Ligated with pSB1A2:
  • plac+rbs
  • GFP+tt
  • GFPf+tt
  • Transformed DB3.1 cells with 5μl ligation reaction


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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