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Team:Hawaii/Notebook/2008-08- 2
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
PCR
- Grace
- Colony PCR of nir-1, nir-2, GFPf-1, GFPf-2
- Annealed at 62C; extended for 1 min.
- Used all new PCR water, primer solution, and Green Taq
- Cut out bands for nir-1, (correct size)
- PCR of PCR components to test for contamination
- New primer solution + new PCR water + old Taq = no bands
- Old primer solution + new PCR water + new Taq = band at 300bp = primer solution was contaminated
- New primer solution + old PCR water + new Taq = bands at 270bp and 350bp = PCR water was contaminated
Cryostocked
- Grace
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
