Team:Hawaii/Notebook/2008-09-23
From 2008.igem.org
Revision as of 06:19, 25 September 2008 by Normanwang (Talk | contribs)
Projects | Events | Resources | ||
---|---|---|---|---|
Sponsors | Experiments | Milestones | Protocols | |
Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Plasmid prep
- Grace
- pSB1A3
- rbs+GFPf+tt #1, 2
- nir+rbs+GFP #1, 4, 7
- nir+rbs+slr1+GFPf #2, 8
- nir+rbs+pilA #18
Sequencing
- Margaret
- PCR of rep, ran a gel to verify size, and exo-sap. will send in tomorrow
PCR: pRL1383a MCS Replacement
- Norman
- x6 parallel MCS replacements fragment by PCR amplification. PCR out each MCS replacement insert with HindIII-VF2BB_fx._sb.1 and BamHI-VRBB_rx._sb.1
- BBa_I52002 from pSB4A5 (Spring 2008 Plate 1022 1C)
- BBa_I52001 from pSB4T5 (Spring 2008 Plate 1020 1A)
- BBa_I52002 from BBa_I51020 (BBa_I51020 Base Vector from glycerol stock Inventory INV-Z80-R1-A3)
- BBa_P1010 from pSB1A3 (pSB1A3 from glycerol stock Inventory INV-Z80-R1-A3)
- BBa_P1010 from pSB1A7 (pSB1A7 from glycerol stock Inventory INV-Z80-R1-A3)
- BBa_J33207 from pSB1A2 (BBa_J33207 harboring pSB1A2 from plasmid prep)
- x2 (or x3) Controls
- positive control: BBa_K125805 from pSB1A3 (BBa_K125805 harboring pSB1A3 from glycerol stock Inventory INV-Z80-R1-D4)
- negative control: H2O primer set used on 1-7
- negative control: H2O old Bam-VF2BB Hind-VRBB primers