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Team:Hawaii/Notebook/2008-09- 3
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(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Re-replacement of BB-pRL1383a MCS=== :<strong> Grace</strong> [[Image:090308REdigest.jpg|right|thumb|200px|EtBr stained...) |
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[[Image:090308REdigest.jpg|right|thumb|200px|EtBr stained 0.8% agarose gel ran at 60V for ---. Thirty microliters of RE digest reaction were loaded into each well.]] | [[Image:090308REdigest.jpg|right|thumb|200px|EtBr stained 0.8% agarose gel ran at 60V for ---. Thirty microliters of RE digest reaction were loaded into each well.]] | ||
:* Ran last night's RE digest on a gel | :* Ran last night's RE digest on a gel | ||
| - | : | + | ::* BB-pRL1383a band did not show up. Nanodrop said DNA conc. ~329 ng/μl. Added 22μl for rxn (7000+ ng DNA). DNA conc. really wrong. |
| - | :* | + | ::* Cut RE digested J33207 band from gel. |
| - | + | ||
| - | :* | + | |
| - | + | ||
= Discussion = | = Discussion = | ||
Revision as of 21:51, 3 September 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Re-replacement of BB-pRL1383a MCS
- Grace
- Ran last night's RE digest on a gel
- BB-pRL1383a band did not show up. Nanodrop said DNA conc. ~329 ng/μl. Added 22μl for rxn (7000+ ng DNA). DNA conc. really wrong.
- Cut RE digested J33207 band from gel.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
