Team:Hawaii/Notebook/2008-09- 3

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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Re-replacement of BB-pRL1383a MCS=== :<strong> Grace</strong> [[Image:090308REdigest.jpg|right|thumb|200px|EtBr stained...)
(Re-replacement of BB-pRL1383a MCS)
 
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===Re-replacement of BB-pRL1383a MCS===
===Re-replacement of BB-pRL1383a MCS===
:<strong> Grace</strong>
:<strong> Grace</strong>
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[[Image:090308REdigest.jpg|right|thumb|200px|EtBr stained 0.8% agarose gel ran at 60V for ---. Thirty microliters of RE digest reaction were loaded into each well.]]
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[[Image:090308REdigest.png|right|thumb|200px|EtBr stained 0.8% agarose gel ran at 60V for 2 hours. Thirty microliters of RE digest reaction were loaded into each well.]]
:* Ran last night's RE digest on a gel
:* Ran last night's RE digest on a gel
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:* Extracted bands from gel
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::* BB-pRL1383a band did not show up. Nanodrop said DNA conc. ~329 ng/&mu;l. Added 22&mu;l for rxn (7000+ ng DNA). DNA conc. really wrong.
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:* Treated BB-pRL1383a w/ SAP
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::* Cut RE digested J33207 band from gel.
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:* Ligated --&mu;l J33207 w/ --&mu;l BB-pRL1383a
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:* Overnight RE digest of BB-pRL1383a (my prep from 8/29) with HindIII and BamHI
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:* Transformed DH5&alpha; cells using 5 &mu;l ligation reaction
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= Discussion =
= Discussion =

Latest revision as of 02:16, 6 September 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Re-replacement of BB-pRL1383a MCS

Grace
EtBr stained 0.8% agarose gel ran at 60V for 2 hours. Thirty microliters of RE digest reaction were loaded into each well.
  • Ran last night's RE digest on a gel
  • BB-pRL1383a band did not show up. Nanodrop said DNA conc. ~329 ng/μl. Added 22μl for rxn (7000+ ng DNA). DNA conc. really wrong.
  • Cut RE digested J33207 band from gel.
  • Overnight RE digest of BB-pRL1383a (my prep from 8/29) with HindIII and BamHI

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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