"
Team:Hawaii/Notebook/2008-09- 4
From 2008.igem.org
(Difference between revisions)
(→Re-replacement of BB-pRL1383a MCS) |
(→Wetlab work) |
||
| Line 22: | Line 22: | ||
::* Negative controls = C0012V (SAP treated and no SAP treatment) ligated to self, plac + rbs ligated w/o vector | ::* Negative controls = C0012V (SAP treated and no SAP treatment) ligated to self, plac + rbs ligated w/o vector | ||
:* Transformed into DB3.1 cells | :* Transformed into DB3.1 cells | ||
| + | |||
| + | === Cont'd construction of GFP + TT === | ||
| + | :<strong>Krystle</strong> | ||
| + | :* Transformed negative control and ligation product from Tuesday into DB3.1 | ||
== Drylab Work == | == Drylab Work == | ||
Revision as of 20:12, 4 September 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Re-replacement of BB-pRL1383a MCS
- Grace
- Ran last night's RE digest on agarose gel
- Extracted J33207 and BB-pRL1383a from gel
- Treated BB-pRL1383a w/ SAP
- Checked DNA concentration of BB-pRL1383a (---ng/μl)
- Ligated J33207 with BB-pRL1383a
- Negative controls = BB-pRL1383a ligated to self, J33207 ligated to self
- Transformed into DH5α cells
3A construction of plac+rbs
- Grace
- Ligated:
- C0012V (SAP treated) + plac + rbs (B0034 back ligation)
- C0012V + plac + rbs (B0034 back ligation)
- Negative controls = C0012V (SAP treated and no SAP treatment) ligated to self, plac + rbs ligated w/o vector
- Transformed into DB3.1 cells
Cont'd construction of GFP + TT
- Krystle
- Transformed negative control and ligation product from Tuesday into DB3.1
Drylab Work
Name of Task
- name of person/people who performed the task
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
