"
Team:Hawaii/Notebook/2008-09- 6
From 2008.igem.org
Revision as of 06:33, 7 September 2008 by MargaretRuzicka (Talk | contribs)
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Construction of B_H_R Vector
- Margaret
- Yesterday's Restriction Digest
- Plac+B0030 and Plac+B0034: ethanol precipitation http://openwetware.org/wiki/Ethanol_precipitation_of_small_DNA_fragments
- pSB1A3, pSB3K3, J33207: de-phosphorylated using SAP
- Gel Quantification
- Some of these products did not appear on the gel, so other methods will be used to generate them.
- Plac+B0030 and Plac+B0034 did not show up on the gel, so the ligation is being performed tonight, pSB1A3 and pSB3K3 plasmid preps from 9/4 showed a good band.
- Ligation 1:1, followed by Restriction digest with E,S,& P (to cut up any polymers formed and to leave E & S overhang):
- Plac + B0030 (500ng)
- Plac + B0030 (100ng)
- Plac + B0034 (500ng)
- Plac + B0030 (100ng)
- pSB3K3 restriction digested with E, P
OriT test
Margaret
- The biobrick form of OriTr did not show up on the above mentioned gel, so I am:
- PCR amplifying it from the biobrick paper
- Extracting DNA from the biobrick registry (1004, 10D) and transforming into DH5-alpha cells. The negative control is pSB1A3-dephosphorylated, the positive control is pSB1A3, from a plasmid prep.
Culturing
Margaret
- DH5-alpha (for making competent cells)
- rep, J33207, pSB3K3, and omega interposon
Drylab Work
Name of Task
- name of person/people who performed the task
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
