Revision as of 21:52, 13 October 2008

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Things we did today

Grace

EtBr stained 2% agarose gel ran at 95V for 1 hour. Ten microliters of PCR product were loaded into each lane.

Grace

Restreaked 5%LB+BG-11 plates onto BG-11+sp_2.5+sm_2.5 plates for isolation of cyanos
Inoculated BG-11+sp_2.5+sm_2.5 liquid media with conjugated E. coli/cyanos
Incubated at 37C with 2% CO₂ for 11 days. Liquid cultures were shaken at 100 rpm also.

name of person/people who performed the task

Summary of task and what was done. Link to experiment for detailed notes if necessary.
e.g. read through papers, worked on proposal, etc.

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

@@ Line 7: / Line 7: @@
 [[Image:101308colony.jpg|right|thumb|250px|EtBr stained 2% agarose gel ran at 95V for 1 hour. Ten microliters of PCR product were loaded into each lane.]]
 :* Colony PCR of restreak plates
+::* Bands ~600bp. Plasmid did not cut.
+:* Overnight RE digest of plasmid w/ EcoRI and PstI
+===Triparental Conjugation===
+:<strong>Grace</strong>
+:* Restreaked 5%LB+BG-11 plates onto BG-11+sp<sub>2.5</sub>+sm<sub>2.5</sub> plates for isolation of cyanos
+:* Inoculated BG-11+sp<sub>2.5</sub>+sm<sub>2.5</sub> liquid media with conjugated ''E. coli''/cyanos
+:* Incubated at 37C with 2% CO<sub>2</sub> for 11 days. Liquid cultures were shaken at 100 rpm also.
 == Drylab Work ==