Team:Hawaii/Notebook/2008-10-13

From 2008.igem.org

(Difference between revisions)
(Verification of transformants)
(Drylab Work)
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:* Incubated at 37C with 2% CO<sub>2</sub> for 11 days. Liquid cultures were shaken at 100 rpm also.
:* Incubated at 37C with 2% CO<sub>2</sub> for 11 days. Liquid cultures were shaken at 100 rpm also.
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== Drylab Work ==
 
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===Name of Task===
 
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:<strong> name of person/people who performed the task</strong>
 
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:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
 
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:* e.g. read through papers, worked on proposal, etc.
 

Revision as of 21:54, 13 October 2008

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Things we did today

Wetlab work

Verification of transformants

Grace
EtBr stained 2% agarose gel ran at 95V for 1 hour. Ten microliters of PCR product were loaded into each lane.
  • Colony PCR of restreak plates
  • Bands ~600bp. Plasmid did not cut.
  • Overnight RE digest of plasmid w/ EcoRI and PstI

Triparental Conjugation

Grace
  • Restreaked 5%LB+BG-11 plates onto BG-11+sp2.5+sm2.5 plates for isolation of cyanos
  • Inoculated BG-11+sp2.5+sm2.5 liquid media with conjugated E. coli/cyanos
  • Incubated at 37C with 2% CO2 for 11 days. Liquid cultures were shaken at 100 rpm also.


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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