Team:Hawaii/Notebook/2008-10-13
From 2008.igem.org
(Difference between revisions)
(→Verification of transformants) |
(→Drylab Work) |
||
Line 17: | Line 17: | ||
:* Incubated at 37C with 2% CO<sub>2</sub> for 11 days. Liquid cultures were shaken at 100 rpm also. | :* Incubated at 37C with 2% CO<sub>2</sub> for 11 days. Liquid cultures were shaken at 100 rpm also. | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
Revision as of 21:54, 13 October 2008
Projects | Events | Resources | ||
---|---|---|---|---|
Sponsors | Experiments | Milestones | Protocols | |
Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of transformants
- Grace
- Colony PCR of restreak plates
- Bands ~600bp. Plasmid did not cut.
- Overnight RE digest of plasmid w/ EcoRI and PstI
Triparental Conjugation
- Grace
- Restreaked 5%LB+BG-11 plates onto BG-11+sp2.5+sm2.5 plates for isolation of cyanos
- Inoculated BG-11+sp2.5+sm2.5 liquid media with conjugated E. coli/cyanos
- Incubated at 37C with 2% CO2 for 11 days. Liquid cultures were shaken at 100 rpm also.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson