Revision as of 04:23, 14 October 2008

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Things we did today

Grace

EtBr stained 2% agarose gel ran at 95V for 1 hour. Ten microliters of PCR product were loaded into each lane.

EtBr stained 2% agarose gel ran at 60V for 2 hours. Thirty microliters of RE digested plasmid were loaded into each lane.

Krystle

Grace

Grace

Restreaked 5%LB+BG-11 plates onto BG-11+sp_2.5+sm_2.5 plates for isolation of cyanos
Inoculated BG-11+sp_2.5+sm_2.5 liquid media with conjugated E. coli/cyanos
Incubated at 37C with 2% CO₂ for 11 days. Liquid cultures were shaken at 100 rpm also.

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

@@ Line 13: / Line 13: @@
 :* RE digest of prpgt, rgt1 plasmids with EcoRI and PstI
 :* Ran RE digests on gel
+:* Bands did not correspond to what was supposedly loaded
 :<strong>Grace</strong>
-:*Extracted bands from gel
+:* PCR of prpgt #5, 7, 11, nrsg #6, rgt #1
-:* Ligated prpgt7,11, plac and rgt1, nir and rgt1, nrsg6 with BBpRL1383a-1
+:* Overnight RE digest of devices with EcoRI and PstI and rgt#1 with XbaI and PstI
-:* Ligated J33207 and pRL1383a
-:* Used 5 &mu;l to transform DB3.1 cells
 ===Triparental Conjugation===