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Team:Hawaii/Notebook/2008-10-13
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of transformants
- Grace
- Colony PCR of restreak plates
- Bands ~600bp. Plasmid did not cut.
File:101308REdigest.jpg
EtBr stained 2% agarose gel ran at 60V for 2 hours. Thirty microliters of RE digested plasmid were loaded into each lane.
- Krystle
- RE digest of plasmid w/ EcoRI and PstI
- RE digest of prpgt, rgt1 plasmids with EcoRI and PstI
- Ran RE digests on gel
- Grace
- Extracted bands from gel
- Ligated prpgt7,11, plac and rgt1, nir and rgt1, nrsg6 with BBpRL1383a-1
- Ligated J33207 and pRL1383a
- Used 5 μl to transform DB3.1 cells
Triparental Conjugation
- Grace
- Restreaked 5%LB+BG-11 plates onto BG-11+sp2.5+sm2.5 plates for isolation of cyanos
- Inoculated BG-11+sp2.5+sm2.5 liquid media with conjugated E. coli/cyanos
- Incubated at 37C with 2% CO2 for 11 days. Liquid cultures were shaken at 100 rpm also.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
