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Team:Hawaii/Notebook/2008-10-14
From 2008.igem.org
(Difference between revisions)
(→Construction of secretion device (cont.)) |
(→Wetlab work) |
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::* nrsg6 (from 10/11) + BBpRL1383a-1 | ::* nrsg6 (from 10/11) + BBpRL1383a-1 | ||
::* J33207 + pRL1383a | ::* J33207 + pRL1383a | ||
| + | |||
| + | ===Plasmid Prep=== | ||
| + | :<strong>Krystle</strong> | ||
| + | :* Plasmid Prepped 2 cultures of BBpRL1383 | ||
| + | :* Nanodrop concentration is 330 ng/ul | ||
| + | :* Gel verification for plasmid shows blank lanes | ||
==Drylab Work== | ==Drylab Work== | ||
Latest revision as of 00:57, 30 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Construction of secretion device (cont.)
- Grace
- Ran RE digests on gel
- Extracted rgt1 and prpgt5 from gel
- Ligated:
- prpgt5 + BBpRL1383a-1
- nir + rgt1 + BBpRL1383a-1
- plac + rgt1 + BBpRL1383a-1
- nrsg6 (from 10/11) + BBpRL1383a-1
- J33207 + pRL1383a
Plasmid Prep
- Krystle
- Plasmid Prepped 2 cultures of BBpRL1383
- Nanodrop concentration is 330 ng/ul
- Gel verification for plasmid shows blank lanes
Drylab Work
Sequencing analysis
- Grace
- prpgt, nrsg, sgt, and pgt sequences all returned inserts of E. coli genomic DNA instead of desired construct
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
