Team:Hawaii/Notebook/2008-10-20

From 2008.igem.org

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(Made LB+sp+sm plates for antibiotic gradient test on Wednesday)
(Wetlab work)
 
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===Large scale plasmid prep of BB-pRL1383a (blue)===
===Large scale plasmid prep of BB-pRL1383a (blue)===
:<strong>Grace</strong>
:<strong>Grace</strong>
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-
 
===PCR of J04430 (lac+rbs+GFP+tt+tt)===
===PCR of J04430 (lac+rbs+GFP+tt+tt)===
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:* EcoRI and PstI digest of BBpRL1383a and J04430
:* EcoRI and PstI digest of BBpRL1383a and J04430
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===Made LB+sp+sm plates for antibiotic gradient test on Wednesday===
+
===Made LB+sp<sub>100</sub> plates ===
:<strong>Grace </strong>
:<strong>Grace </strong>
-
:* 30 ml plates
+
===Prepped for sequencing===
 +
:<strong>Krystle</strong>
 +
:* Sent out correct-sized product to CORE for sequencing
 +
::* nir+rbs
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::* slr+gfpf
 +
 
 +
===Construction of GFP secretion device===
 +
:<strong>Krystle</strong>
 +
:* Restriction digested additional PCR product
 +
::*nir+rbs with EcoRI and SpeI
= Discussion =
= Discussion =

Latest revision as of 01:02, 30 October 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Large scale plasmid prep of BB-pRL1383a (blue)

Grace

PCR of J04430 (lac+rbs+GFP+tt+tt)

Grace
  • Will act as (-) control for GFP secretion in cyanos

Overnight RE digest

Grace
  • EcoRI and PstI digest of BBpRL1383a and J04430

Made LB+sp100 plates

Grace

Prepped for sequencing

Krystle
  • Sent out correct-sized product to CORE for sequencing
  • nir+rbs
  • slr+gfpf

Construction of GFP secretion device

Krystle
  • Restriction digested additional PCR product
  • nir+rbs with EcoRI and SpeI

Discussion

Quote of the Day

Garbage truck dripping E. coli... this has got to stop. - Roswell Mayor, http://www.youtube.com/watch?v=f7mEb-p4F44 (ok maybe we should start taking Amp + Kan + Sm + Sp before going to the bathroom)


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