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Team:Hawaii/Notebook/2008-10-24
From 2008.igem.org
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:* Observations collected 19 hours after initial plating. | :* Observations collected 19 hours after initial plating. | ||
| + | [[Image:glowcolony.jpg|right|thumb|Restreak plate of colonies 1, 2 and 3 of the lac+rbs_slr+gfpf construct.]] | ||
===Verification of GFP secretion device=== | ===Verification of GFP secretion device=== | ||
:<strong>Krystle</strong> | :<strong>Krystle</strong> | ||
:*Viewed restreaks of lac+rbs+slr+gfgf and nir+rbs+slr+gfpf under blue flourescent light. | :*Viewed restreaks of lac+rbs+slr+gfgf and nir+rbs+slr+gfpf under blue flourescent light. | ||
:*lac+rbs+slr+gfpf colony 1 glows! | :*lac+rbs+slr+gfpf colony 1 glows! | ||
| - | |||
==Dry Lab Work== | ==Dry Lab Work== | ||
Latest revision as of 01:14, 30 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Prep for sequencing BB1
- Grace
- ExoSAP'd BB1 PCr product and sent to CORE Hawaii for sequencing
Plasmid prep of parts to send to iGEM
- Grace
| Plasmid | Concentration |
|---|---|
| nir | |
| slr | |
| pilA | |
| GFPf | |
| nir+rbs | |
| slr+GFPf | |
| BBpRL1383a #1 from sp100 plate |
Antibiotic test
- Grace
| Plate | Colony | Growth? | Blue? |
|---|---|---|---|
| LB+amp100 | sp2.5 blue | No | No |
| sp100 #1 | No | No | |
| sp100 #2 | No | No | |
| LB | sp2.5 blue | Yes | Yes, ~20 |
| sp100 #1 | Yes | No | |
| sp100 #2 | Yes | No | |
| LB+sp100 | sp2.5 blue | No | No |
| sp100 #1 | Yes | No | |
| sp100 #2 | Yes | No |
- Observations collected 19 hours after initial plating.
Verification of GFP secretion device
- Krystle
- Viewed restreaks of lac+rbs+slr+gfgf and nir+rbs+slr+gfpf under blue flourescent light.
- lac+rbs+slr+gfpf colony 1 glows!
Dry Lab Work
Analyzed sequencing results
- Krystle
- Sequencing for lac+rbs+slr+gfpf only had a good reverse read, must be resequenced
- All sequenced nir+rbs+slr+gfpf colonies contain the incorrect insert
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
