Team:Hawaii/Notebook/2008-10-24

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Things we did today

Wetlab work

Prep for sequencing BB1

Grace
  • ExoSAP'd BB1 PCr product and sent to CORE Hawaii for sequencing

Plasmid prep of parts to send to iGEM

Grace
Plasmid Concentration
nir
slr
pilA
GFPf
nir+rbs
slr+GFPf
BBpRL1383a #1 from sp100 plate

Antibiotic test

Grace
Restreak of transformants
Plate Colony Growth? Blue?
LB+amp100 sp2.5 blue NoNo
sp100 #1NoNo
sp100 #2NoNo
LBsp2.5 blue YesYes, ~20
sp100 #1YesNo
sp100 #2YesNo
LB+sp100sp2.5 blue NoNo
sp100 #1YesNo
sp100 #2YesNo


  • Observations collected 19 hours after initial plating.

Verification of GFP secretion device

Krystle
  • Viewed restreaks of lac+rbs+slr+gfgf and nir+rbs+slr+gfpf under blue flourescent light.
  • lac+rbs+slr+gfpf colony 1 glows!
Restreak plate of colonies 1, 3 and 4 of the lac+rbs_slr+gfpf construct.

Dry Lab Work

Analyzed sequencing results

Krystle
  • Sequencing for lac+rbs+slr+gfpf only had a good reverse read, must be resequenced
  • All sequenced nir+rbs+slr+gfpf colonies contain the incorrect insert

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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