Team:Hawaii/Notebook/2008-10-25

From 2008.igem.org

(Difference between revisions)
(Verification of plasmid preps)
(Wetlab work)
 
Line 11: Line 11:
===Inoculated 50ml TB+sp<sub>100</sub> with single colony BB1 for plasmid prep===
===Inoculated 50ml TB+sp<sub>100</sub> with single colony BB1 for plasmid prep===
:<strong>Grace</strong>
:<strong>Grace</strong>
 +
 +
===Construction of BB-pRL1383a/K125820===
 +
<strong>Krystle</strong>
 +
:* Restriction digest BB-pRL1383a with EcoRI and PstI
 +
:* Dephosphorelated restriction product with SAP for 1 hour
 +
:* Ligated cut/dephosphorelated product with gel purified K125820 using T4 ligase
== Drylab Work ==
== Drylab Work ==

Latest revision as of 00:21, 30 October 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Verification of plasmid preps

Grace
EtBr stained 4% agarose gel ran at 60V for 2 hours.
  • PCR'd plasmid preps from yesterday
  • RE digested all preps using EcoRI and PstI for 3 hours
  • Ran on gel

Inoculated 50ml TB+sp100 with single colony BB1 for plasmid prep

Grace

Construction of BB-pRL1383a/K125820

Krystle

  • Restriction digest BB-pRL1383a with EcoRI and PstI
  • Dephosphorelated restriction product with SAP for 1 hour
  • Ligated cut/dephosphorelated product with gel purified K125820 using T4 ligase

Drylab Work

iGEM presentation

Grace
  • Worked on presentation

parts

Margaret
  • added parts to registry

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]