Team:Hawaii/Notebook/2008-10-25

From 2008.igem.org

(Difference between revisions)
(Verification of plasmid preps)
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:* RE digested all preps using EcoRI and PstI for 3 hours
:* RE digested all preps using EcoRI and PstI for 3 hours
:* Ran on gel
:* Ran on gel
 +
===Inoculated 50ml TB+sp<sub>100</sub> with single colony BB1 for plasmid prep===
 +
:<strong>Grace</strong>
== Drylab Work ==
== Drylab Work ==

Revision as of 21:19, 26 October 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Verification of plasmid preps

Grace
EtBr stained 4% agarose gel ran at 60V for 2 hours.
  • PCR'd plasmid preps from yesterday
  • RE digested all preps using EcoRI and PstI for 3 hours
  • Ran on gel

Inoculated 50ml TB+sp100 with single colony BB1 for plasmid prep

Grace

Drylab Work

iGEM presentation

Grace
  • Worked on presentation

parts

Margaret
  • added parts to registry

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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