"
Team:Hawaii/Notebook/2008-10-26
From 2008.igem.org
(Difference between revisions)
(→Plasmid prep) |
(→Plasmid prep) |
||
| Line 4: | Line 4: | ||
== Wetlab work == | == Wetlab work == | ||
===Plasmid prep=== | ===Plasmid prep=== | ||
| - | |||
[[Image:102608plasmid.png|right|thumb|EtBr stained 0.8% agarose gel ran at 95V for 1 hour. Ten microliters of plasmid were loaded into each well.]] | [[Image:102608plasmid.png|right|thumb|EtBr stained 0.8% agarose gel ran at 95V for 1 hour. Ten microliters of plasmid were loaded into each well.]] | ||
| + | :<strong> Grace</strong> | ||
| + | |||
:* Used Qiaprep Spin Miniprep Kit to plasmid prep BB1 (10/22 and 10/25 inoculations) | :* Used Qiaprep Spin Miniprep Kit to plasmid prep BB1 (10/22 and 10/25 inoculations) | ||
:* Ran on gel to verify plasmid | :* Ran on gel to verify plasmid | ||
Revision as of 21:56, 26 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Plasmid prep
- Grace
- Used Qiaprep Spin Miniprep Kit to plasmid prep BB1 (10/22 and 10/25 inoculations)
- Ran on gel to verify plasmid
- ~2ng/μl
- The prep kit yields really clean preps in ~1 hour. :D!
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
