Team:Hawaii/Notebook/2008-10-26

From 2008.igem.org

(Difference between revisions)
(→Things we did today)
(→Wetlab work)
Line 17: Line 17:
===Construction of BB-pRL1383a/K125820===
===Construction of BB-pRL1383a/K125820===
-
<strong>Krystle</strong>
+
:<strong>Krystle</strong>
:* Transformation of overnight ligation products
:* Transformation of overnight ligation products
:* Plated onto LB sp<sub>100</sub> plates
:* Plated onto LB sp<sub>100</sub> plates

Revision as of 01:15, 30 October 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Plasmid prep

EtBr stained 0.8% agarose gel ran at 95V for 1 hour. Ten microliters of plasmid were loaded into each well.
Grace
  • Used Qiaprep Spin Miniprep Kit to plasmid prep BB1 (10/22 and 10/25 inoculations)
  • Ran on gel to verify plasmid
  • ~2ng/μl
  • The prep kit yields really clean preps in ~1 hour. :D!

Plasmid prep

EtBr stained 0.8% agarose gel ran at 95V for 1 hour. 5 microliters of plasmid were loaded into wells 3 and 4 while ligation products were loaded into lanes 5 and 6.
Margaret

Construction of BB-pRL1383a/K125820

Krystle
  • Transformation of overnight ligation products
  • Plated onto LB sp100 plates

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


Sponsor_UHM.gifSponsor_OVCRGE.gifSponsor_CTAHR.gif


Retrieved from "http://2008.igem.org/Team:Hawaii/Notebook/2008-10-26"