Team:Hawaii/Notebook/2008-10-26

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Things we did today

Wetlab work

Plasmid prep

EtBr stained 0.8% agarose gel ran at 95V for 1 hour. Ten microliters of plasmid were loaded into each well.
Grace
  • Used Qiaprep Spin Miniprep Kit to plasmid prep BB1 (10/22 and 10/25 inoculations)
  • Ran on gel to verify plasmid
  • ~2ng/μl
  • The prep kit yields really clean preps in ~1 hour. :D!

Plasmid prep

EtBr stained 0.8% agarose gel ran at 95V for 1 hour. 5 microliters of plasmid were loaded into wells 3 and 4 while ligation products were loaded into lanes 5 and 6.
Margaret

Construction of BB-pRL1383a/K125820

Krystle
  • Transformation of overnight ligation products
  • Plated onto LB sp100 plates

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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