Team:Hawaii/Parts

From 2008.igem.org

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! width="250"|Description
! width="250"|Description
! width="75"|Type
! width="75"|Type
-
|- style="background:#f0f0f0; color:black"
 
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125000 '''K125000''']
 
-
| pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (rbs)
 
-
| plasmid
 
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125010 '''K125010''']
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125010 '''K125010''']
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| plasmid
| plasmid
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125020 '''K125020''']
+
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125330 '''K125330''']
-
| rep/oriv/aada/orit
+
| P1 lytic replication
-
| intermediate for RSF1010 derived plasmid
+
|
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125340 '''K125340''']
 +
| oriV
 +
|
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125510 '''K125510''']
 +
| Plasmid replication proteins
 +
| protein coding
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125520 '''K125520''']
 +
| aadA gene
 +
| protein coding
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125800 '''K125800''']
 +
| rep proteins with promoter and rbs
 +
| protein coding
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125830 '''K125830''']
 +
| ''Synechocystis''-specific, nitrate-inducible GFP secretion device under the control of a ''nirA'' promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_K125100 K125100]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500])
 +
| device
 +
|}
 +
 
 +
==Parts submitted==
 +
{| style="background:#BCEE68;color:green;" border="0" cellpadding="10" cellspacing="0" align="center"
 +
|-
 +
! width="75"|Part number
 +
! width="250"|Description
 +
! width="75"|Type
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125000 '''K125000''']
 +
| pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (''lac'' promoter+''lacZ'')
 +
| plasmid
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125100 '''K125100''']
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125100 '''K125100''']
Line 54: Line 82:
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125110 '''K125110''']
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125110 '''K125110''']
| nitrate inducible ''nir'' promoter + ribosome binding site with efficiency of 0.6 relative to [http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030]
| nitrate inducible ''nir'' promoter + ribosome binding site with efficiency of 0.6 relative to [http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030]
-
| regulator
 
-
|- style="background:#f0f0f0; color:black"
 
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125110 '''K125120''']
 
-
| ''lac'' promoter + ribosome binding site with efficiency of 1.0 (composite of I14032 and B0030)
 
| regulator
| regulator
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
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| protein coding
| protein coding
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125320 '''K125320''']
+
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125500 '''K125500''']
-
| oriT derived from RP4
+
| GFP fusion protein
-
| origin of transfer
+
| protein coding
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125810 '''K125810''']
 +
| N-terminal slr2016 signal sequence attached to GFP fusion protein (Composite of [http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500])
 +
| protein coding
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125820 '''K125820''']
 +
| ''Synechocystis''-specific constitutive GFP secretion device under the control of a ''lac'' promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_I14032 I14032]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0034 B0034][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500])
 +
| device
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125350 '''K125350''']
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125350 '''K125350''']
Line 80: Line 112:
| conjugation screening
| conjugation screening
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125500 '''K125500''']
+
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125320 '''K125320''']
-
| GFP fusion protein
+
| oriT derived from RP4
-
| protein coding
+
| origin of transfer
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125520 '''K125520''']
+
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125020 '''K125020''']
-
| aadA gene
+
| rep/oriv/aada/orit
-
| protein coding
+
| intermediate for RSF1010 derived plasmid
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125530 '''K125530''']
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125530 '''K125530''']
| aadA gene with promoter and rbs
| aadA gene with promoter and rbs
| protein coding
| protein coding
-
|- style="background:#f0f0f0; color:black"
 
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125800 '''K125800''']
 
-
| rep proteins with promoter and rbs
 
-
| protein coding
 
-
|- style="background:#f0f0f0; color:black"
 
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125810 '''K125810''']
 
-
| N-terminal slr2016 signal sequence attached to GFP fusion protein
 
-
| protein coding
 
-
|- style="background:#f0f0f0; color:black"
 
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125820 '''K125820''']
 
-
| ''Synechocystis''-specific constitutive GFP secretion device under the control of a ''lac'' promoter
 
-
| device
 
|}
|}
-
 
-
==Parts submitted==
 

Latest revision as of 03:53, 30 October 2008

Contents

Guidelines for naming parts

Part number Part type
K125000-K125099 plasmid
K125100-K125199 promoter
K125200-K125299 ribosome binding site
K125300-K125499 signal sequences, conjugation sequences, random stuff
K125500-K125699 protein coding
K125700-K125799 transcription terminator
K125800-K125999 devices

Parts planned

Parts in process (building)

Part number Description Type
K125010 Broad-Host-Range plasmid derived from RSF1010 plasmid
K125330 P1 lytic replication
K125340 oriV
K125510 Plasmid replication proteins protein coding
K125520 aadA gene protein coding
K125800 rep proteins with promoter and rbs protein coding
K125830 Synechocystis-specific, nitrate-inducible GFP secretion device under the control of a nirA promoter (K125100+B0030K125310+K125500) device

Parts submitted

Part number Description Type
K125000 pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (lac promoter+lacZ) plasmid
K125100 nitrate inducible nir promoter regulator
K125110 nitrate inducible nir promoter + ribosome binding site with efficiency of 0.6 relative to B0030 regulator
K125300 cyanobacterial pilA derived amino termninus protein secretion signal sequence protein coding
K125310 cyanobacterial slr2016 derived amino terminus protein secretion signal sequence protein coding
K125500 GFP fusion protein protein coding
K125810 N-terminal slr2016 signal sequence attached to GFP fusion protein (Composite of K125310+K125500) protein coding
K125820 Synechocystis-specific constitutive GFP secretion device under the control of a lac promoter (I14032+B0034K125310+K125500) device
K125350 oriTr/LacZ conjugation screening
K125360 oriT/LacZ conjugation screening
K125320 oriT derived from RP4 origin of transfer
K125020 rep/oriv/aada/orit intermediate for RSF1010 derived plasmid
K125530 aadA gene with promoter and rbs protein coding
Retrieved from "http://2008.igem.org/Team:Hawaii/Parts"