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Team:Hawaii/Parts
From 2008.igem.org
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| pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (rbs) | | pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (rbs) | ||
| plasmid | | plasmid | ||
| + | |- style="background:#f0f0f0; color:black" | ||
| + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125010 '''K125010'''] | ||
| + | | Broad-Host-Range plasmid derived from RSF1010 | ||
| + | | plasmid | ||
| + | |- style="background:#f0f0f0; color:black" | ||
| + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125020 '''K125020'''] | ||
| + | | rep/oriv/aada/orit | ||
| + | | intermediate for RSF1010 derived plasmid | ||
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125100 '''K125100'''] | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125100 '''K125100'''] | ||
| Line 59: | Line 67: | ||
| cyanobacterial slr2016 derived amino terminus protein secretion signal sequence | | cyanobacterial slr2016 derived amino terminus protein secretion signal sequence | ||
| protein coding | | protein coding | ||
| + | |- style="background:#f0f0f0; color:black" | ||
| + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125320 '''K125320'''] | ||
| + | | oriT derived from RP4 | ||
| + | | origin of transfer | ||
| + | |- style="background:#f0f0f0; color:black" | ||
| + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125350 '''K125350'''] | ||
| + | | oriTr/LacZ | ||
| + | | conjugation screening | ||
| + | |- style="background:#f0f0f0; color:black" | ||
| + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125360 '''K125360'''] | ||
| + | | oriT/LacZ | ||
| + | | conjugation screening | ||
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125500 '''K125500'''] | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125500 '''K125500'''] | ||
| Line 64: | Line 84: | ||
| protein coding | | protein coding | ||
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
| - | + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125520 '''K125520'''] | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125520 ''' | + | |
| aadA gene | | aadA gene | ||
| protein coding | | protein coding | ||
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
| - | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125530 ''' | + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125530 '''K125530'''] |
| aadA gene with promoter and rbs | | aadA gene with promoter and rbs | ||
| protein coding | | protein coding | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125800 '''K125800'''] | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125800 '''K125800'''] | ||
| Line 88: | Line 96: | ||
| protein coding | | protein coding | ||
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
| - | | [http://partsregistry.org/wiki/index.php/Part: | + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125810 '''K125810'''] |
| - | | | + | | N-terminal slr2016 signal sequence attached to GFP fusion protein |
| - | | | + | | protein coding |
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
| - | | [http://partsregistry.org/wiki/index.php/Part: | + | | [http://partsregistry.org/wiki/index.php/Part:BBa_K125820 '''K125820'''] |
| - | | | + | | ''Synechocystis''-specific constitutive GFP secretion device under the control of a ''lac'' promoter |
| - | + | | device | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | | | + | |
|} | |} | ||
==Parts submitted== | ==Parts submitted== | ||
Revision as of 22:04, 29 October 2008
Contents |
Guidelines for naming parts
| Part number | Part type |
|---|---|
| K125000-K125099 | plasmid |
| K125100-K125199 | promoter |
| K125200-K125299 | ribosome binding site |
| K125300-K125499 | signal sequences, conjugation sequences, random stuff |
| K125500-K125699 | protein coding |
| K125700-K125799 | transcription terminator |
| K125800-K125999 | devices |
Parts planned
Parts in process (building)
| Part number | Description | Type |
|---|---|---|
| K125000 | pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (rbs) | plasmid |
| K125010 | Broad-Host-Range plasmid derived from RSF1010 | plasmid |
| K125020 | rep/oriv/aada/orit | intermediate for RSF1010 derived plasmid |
| K125100 | nitrate inducible nir promoter | regulator |
| K125110 | nitrate inducible nir promoter + ribosome binding site with efficiency of 0.6 relative to B0030 | regulator |
| K125120 | lac promoter + ribosome binding site with efficiency of 1.0 (composite of I14032 and B0030) | regulator |
| K125300 | cyanobacterial pilA derived amino termninus protein secretion signal sequence | protein coding |
| K125310 | cyanobacterial slr2016 derived amino terminus protein secretion signal sequence | protein coding |
| K125320 | oriT derived from RP4 | origin of transfer |
| K125350 | oriTr/LacZ | conjugation screening |
| K125360 | oriT/LacZ | conjugation screening |
| K125500 | GFP fusion protein | protein coding |
| K125520 | aadA gene | protein coding |
| K125530 | aadA gene with promoter and rbs | protein coding |
| K125800 | rep proteins with promoter and rbs | protein coding |
| K125810 | N-terminal slr2016 signal sequence attached to GFP fusion protein | protein coding |
| K125820 | Synechocystis-specific constitutive GFP secretion device under the control of a lac promoter | device |
