Team:Hawaii/Parts

From 2008.igem.org

(Difference between revisions)
(Parts in process (building))
(Parts in process (building))
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| pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (rbs)
| pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (rbs)
| plasmid
| plasmid
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125010 '''K125010''']
 +
| Broad-Host-Range plasmid derived from RSF1010
 +
| plasmid
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125020 '''K125020''']
 +
| rep/oriv/aada/orit
 +
| intermediate for RSF1010 derived plasmid
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125100 '''K125100''']
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125100 '''K125100''']
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| cyanobacterial slr2016 derived amino terminus protein secretion signal sequence
| cyanobacterial slr2016 derived amino terminus protein secretion signal sequence
| protein coding
| protein coding
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125320 '''K125320''']
 +
| oriT derived from RP4
 +
| origin of transfer
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125350 '''K125350''']
 +
| oriTr/LacZ
 +
| conjugation screening
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125360 '''K125360''']
 +
| oriT/LacZ
 +
| conjugation screening
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125500 '''K125500''']
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125500 '''K125500''']
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| protein coding
| protein coding
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125810 '''K128510''']
+
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125520 '''K125520''']
-
| N-terminal slr2016 signal sequence attached to GFP fusion protein
+
-
| protein coding
+
-
|- style="background:#f0f0f0; color:black"
+
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125520 '''K128520''']
+
| aadA gene
| aadA gene
| protein coding
| protein coding
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125530 '''K128530''']
+
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125530 '''K125530''']
| aadA gene with promoter and rbs
| aadA gene with promoter and rbs
| protein coding
| protein coding
-
|- style="background:#f0f0f0; color:black"
 
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125010 '''K125010''']
 
-
| Broad-Host-Range plasmid derived from RSF1010
 
-
| plasmid
 
-
|- style="background:#f0f0f0; color:black"
 
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125320 '''K125320''']
 
-
| oriT derived from RP4
 
-
| origin of transfer
 
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125800 '''K125800''']
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125800 '''K125800''']
Line 88: Line 96:
| protein coding
| protein coding
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125350 '''K125350''']
+
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125810 '''K125810''']
-
| oriTr/LacZ
+
| N-terminal slr2016 signal sequence attached to GFP fusion protein
-
| conjugation screening
+
| protein coding
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125360 '''K125360''']
+
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125820 '''K125820''']
-
| oriT/LacZ
+
| ''Synechocystis''-specific constitutive GFP secretion device under the control of a ''lac'' promoter
-
| conjugation screening
+
| device
-
|- style="background:#f0f0f0; color:black"
+
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125020 '''K125020''']
+
-
| rep/oriv/aada/orit
+
-
| intermediate for RSF1010 derived plasmid
+
|}
|}
==Parts submitted==
==Parts submitted==

Revision as of 22:04, 29 October 2008

Contents

Guidelines for naming parts

Part number Part type
K125000-K125099 plasmid
K125100-K125199 promoter
K125200-K125299 ribosome binding site
K125300-K125499 signal sequences, conjugation sequences, random stuff
K125500-K125699 protein coding
K125700-K125799 transcription terminator
K125800-K125999 devices

Parts planned

Parts in process (building)

Part number Description Type
[http://partsregistry.org/wiki/index.php/Part:BBa_K125000 K125000] pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (rbs) plasmid
[http://partsregistry.org/wiki/index.php/Part:BBa_K125010 K125010] Broad-Host-Range plasmid derived from RSF1010 plasmid
[http://partsregistry.org/wiki/index.php/Part:BBa_K125020 K125020] rep/oriv/aada/orit intermediate for RSF1010 derived plasmid
[http://partsregistry.org/wiki/index.php/Part:BBa_K125100 K125100] nitrate inducible nir promoter regulator
[http://partsregistry.org/wiki/index.php/Part:BBa_K125110 K125110] nitrate inducible nir promoter + ribosome binding site with efficiency of 0.6 relative to [http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030] regulator
[http://partsregistry.org/wiki/index.php/Part:BBa_K125110 K125120] lac promoter + ribosome binding site with efficiency of 1.0 (composite of I14032 and B0030) regulator
[http://partsregistry.org/wiki/index.php/Part:BBa_K125300 K125300] cyanobacterial pilA derived amino termninus protein secretion signal sequence protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310] cyanobacterial slr2016 derived amino terminus protein secretion signal sequence protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125320 K125320] oriT derived from RP4 origin of transfer
[http://partsregistry.org/wiki/index.php/Part:BBa_K125350 K125350] oriTr/LacZ conjugation screening
[http://partsregistry.org/wiki/index.php/Part:BBa_K125360 K125360] oriT/LacZ conjugation screening
[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500] GFP fusion protein protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125520 K125520] aadA gene protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125530 K125530] aadA gene with promoter and rbs protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125800 K125800] rep proteins with promoter and rbs protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125810 K125810] N-terminal slr2016 signal sequence attached to GFP fusion protein protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125820 K125820] Synechocystis-specific constitutive GFP secretion device under the control of a lac promoter device

Parts submitted