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Team:Heidelberg/Notebook/Sensing Group/Notebook/2ndweek
From 2008.igem.org
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== Tuesday, 08/12/2008 == | == Tuesday, 08/12/2008 == | ||
* Miniprep of correctly sequenced LuxS and Glycerol-stock | * Miniprep of correctly sequenced LuxS and Glycerol-stock | ||
| - | * | + | * LuxQ no. 3 checked via digestion with NcoI/BamHI --> negative result |
| + | * PCR for LuxQ, LuxQ-1, LuxQ-2, Tar-1, Tar-2 with Taq Mastermix | ||
| + | * preparation of O/N culture for LuxQ no. 3 | ||
== Wednesday, 08/13/2008 == | == Wednesday, 08/13/2008 == | ||
Revision as of 10:46, 26 October 2008
Contents |
Monday, 08/11/2008
- preparation of LuxS harboring cells O/N culture
Tuesday, 08/12/2008
- Miniprep of correctly sequenced LuxS and Glycerol-stock
- LuxQ no. 3 checked via digestion with NcoI/BamHI --> negative result
- PCR for LuxQ, LuxQ-1, LuxQ-2, Tar-1, Tar-2 with Taq Mastermix
- preparation of O/N culture for LuxQ no. 3
