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Team:Heidelberg/Notebook/Sensing Group/Notebook/2ndweek
From 2008.igem.org
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* Fusion-PCR for Fusion-1 and repetetion of PCR for LuxQ-2 and LuxQ | * Fusion-PCR for Fusion-1 and repetetion of PCR for LuxQ-2 and LuxQ | ||
[[Image:HD 080813-LuxQ PCR-pDK48 mini LuxQ3 xbaI.png|center|thumb|500px|Fusion-PCR for Fusion-1, LuxQ PCR, LuxQ-2 PCR, pDK48 miniprep and xbaI digestion of LuxQ no. 3]] | [[Image:HD 080813-LuxQ PCR-pDK48 mini LuxQ3 xbaI.png|center|thumb|500px|Fusion-PCR for Fusion-1, LuxQ PCR, LuxQ-2 PCR, pDK48 miniprep and xbaI digestion of LuxQ no. 3]] | ||
| + | * digestion of pDK48 and Fusion-1 with NcoI/NdeI (0.5 µl) and AgeI (1 µl) in NEBuffer 4 | ||
| + | [[Image:HD 080813-F1 pDK48 Digestion.png|center|thumb|200px|Digestion of Fusion-1 and pDK48 with NcoI/NdeI/AgeI]] | ||
== Thursday, 08/14/2008 == | == Thursday, 08/14/2008 == | ||
Revision as of 10:54, 26 October 2008
Contents |
Monday, 08/11/2008
- preparation of LuxS harboring cells O/N culture
Tuesday, 08/12/2008
- Miniprep of correctly sequenced LuxS and Glycerol-stock
- LuxQ no. 3 checked via digestion with NcoI/BamHI --> negative result
- PCR for LuxQ, LuxQ-1, LuxQ-2, Tar-1, Tar-2 with Taq Mastermix
- preparation of O/N culture for LuxQ no. 3
Wednesday, 08/13/2008
- PCR-purification of the 5 PCR-products eluted in 50 µl H2O
- Miniprep of LuxQ no. 3 O/N culture and digestion with xbaI
- Fusion-PCR for Fusion-1 and repetetion of PCR for LuxQ-2 and LuxQ
- digestion of pDK48 and Fusion-1 with NcoI/NdeI (0.5 µl) and AgeI (1 µl) in NEBuffer 4
