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Team:Heidelberg/Notebook/Sensing Group/Notebook/9thweek
From 2008.igem.org
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* Miniprep of Fusion-LuxP-pBAD33 (both constructs) | * Miniprep of Fusion-LuxP-pBAD33 (both constructs) | ||
* Digestion of pSB1A3 with EcoRI,PstI,NdeI (3µl DNA, 40 µl total volume) | * Digestion of pSB1A3 with EcoRI,PstI,NdeI (3µl DNA, 40 µl total volume) | ||
| - | * Digestion of pSB2K3 (10µl DNA, 20µl total volume) with EcoRI,PstI | + | * Digestion of pSB2K3 (10µl DNA, 20µl total volume) with EcoRI,PstI |
| + | [[Image:HD 081001-F-PCR prefix speI.png|center|thumb|400px|PCR GeneArt Fusion constructs with Prefix/Suffix primer yielded no product. F1/F2 with prefix/speI primer were positive, but mutation strategy dropped later. A mix of LuxS and pDK6 was added on the gel to check if double transformation was successfull. Digestions of pSB1A3 and pSB2K3 with EcoRI/Pst were supposed to be used for subsequent standardization of parts]] | ||
== Thursday, 10/02/2008 == | == Thursday, 10/02/2008 == | ||
Revision as of 19:28, 26 October 2008
Contents |
Tuesday, 09/30/2008
- Miniprep of LuxS+pDK6 and F1/F2-Luxp-pBAD33
- Digestion of GeneArt Fusion constructs with EcoRI/PstI and Gel-Extraction
- Digestion of GeneArt Fusion constructs with EcoRI/PstI and Gel-Extraction
Wednesday, 10/01/2008
- Miniprep of Fusion-LuxP-pBAD33 (both constructs)
- Digestion of pSB1A3 with EcoRI,PstI,NdeI (3µl DNA, 40 µl total volume)
- Digestion of pSB2K3 (10µl DNA, 20µl total volume) with EcoRI,PstI
