Team:Heidelberg/Notebook/Visualization/Notebook/1stweek

From 2008.igem.org

(Difference between revisions)
(Monday 01/09/2008)
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==Monday 01/09/2008==
==Monday 01/09/2008==
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* Preparation of TB media with an Agar concentration of 0.4 %, 0.2 % and 0.1 %.
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First microscope test using MG1655 in 6 small chambers from Labtek:
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3 types:
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I - 0.1% agar in LB
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II - 0.25% agar in LB
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III - 0.5% agar in LB
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View:
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I II III
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I II III
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* In I and II each chamber was filled with just 100 µl LB agar to cover the whole ground.
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 +
* Because the III. solution was more viscous 150 µl were necessary to cover the ground.
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* The bacteria were put into one corner of a chamber. (2 µl of a MG1655 overnight culture)
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They have been set under the media or in between.
 +
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* About that another 100 µl of a LB solution with 2 % agar were poured. After the second layer has solidified further 250 µl LB-media were added.
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 +
* The chambers were incubated overnight at 37 °C. For microscope on the next day.
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 +
Notes: Because of surface tensions the solutions tend to be thicker at the borders of the chamber than in the center.
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-> In the further future 150 - 200 µl will be used.
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==Tuesday 02/09/2008==
==Tuesday 02/09/2008==
==Wednesday 03/09/2008==
==Wednesday 03/09/2008==

Revision as of 17:00, 26 October 2008

Overview

Contents

First week

Monday 01/09/2008

  • Preparation of TB media with an Agar concentration of 0.4 %, 0.2 % and 0.1 %.

First microscope test using MG1655 in 6 small chambers from Labtek:

3 types: I - 0.1% agar in LB II - 0.25% agar in LB III - 0.5% agar in LB

View: I II III I II III

  • In I and II each chamber was filled with just 100 µl LB agar to cover the whole ground.
  • Because the III. solution was more viscous 150 µl were necessary to cover the ground.
  • The bacteria were put into one corner of a chamber. (2 µl of a MG1655 overnight culture)

They have been set under the media or in between.

  • About that another 100 µl of a LB solution with 2 % agar were poured. After the second layer has solidified further 250 µl LB-media were added.
  • The chambers were incubated overnight at 37 °C. For microscope on the next day.

Notes: Because of surface tensions the solutions tend to be thicker at the borders of the chamber than in the center. -> In the further future 150 - 200 µl will be used.

Tuesday 02/09/2008

Wednesday 03/09/2008

Thursday 04/09/2008

Friday 05/09/2008

Saturday 06/09/2008

Sunday 07/09/2008

2ndWeek