Team:Heidelberg/Notebook/Visualization/Notebook/SwarmAssays

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Revision as of 16:16, 29 October 2008

Swarm Assays

STARTED 2008-09-27

PURPOSE:
Test of the abbility of strains expressing the fusion receptor, to move against an attractant gradient.

STRAIN:
As autoinducer 2 producing attactant strain DH5α was used.
The fusion receptor plasmid was further cloned into HCB33 and UU1250 strains.

METHOD:
Petri-dishes were used es running chambers.
A petri-dish was filled with a media containig agar. Then the attractant was spotted on premarked positions or spotted as a line through the center of the petri-dish. After the gradient of the attractant has etablished the strains whioch should be attracted by the attractant where also set on premarked positions. The whole petri-dish was incubated at 37 °C and pictures were taken after 24, 48 and 72 hours. For a more detailed protocol visit the methods page.

A few pictures which have been taken during these experiments can be viewed under Projects.

back to the visualization notebook

@@ Line 471: / Line 471: @@
 PURPOSE:<br>
-Test of
+Test of the abbility of strains expressing the fusion receptor, to move against an attractant gradient.
 STRAIN:<br>
+As autoinducer 2 producing attactant strain DH5α was used.<br>
+The fusion receptor plasmid was further cloned into HCB33 and UU1250 strains.<br>
 METHOD:<br>
+Petri-dishes were used es running chambers.<br>
+A petri-dish was filled with a media containig agar. Then the attractant was spotted on premarked positions or spotted as a line through the center of the petri-dish. After the gradient of the attractant has etablished the strains whioch should be attracted by the attractant where also set on premarked positions. The whole petri-dish was incubated at 37 °C and pictures were taken after 24, 48 and 72 hours.
+For a more detailed protocol visit the [https://2008.igem.org/Team:Heidelberg/Notebook/visualization/methods methods] page.