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Team:Heidelberg/Notebook/material
From 2008.igem.org
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| - | == | + | {| class="wikitable" |
| - | + | |- bgcolor=grey; style="height:20px" | |
| + | ! width=200px | || width=250px | || width=250px | | ||
| + | |-align="center" | ||
| + | | SM || 50 mM || Tris, pH 7.5 | ||
| + | |-align="center" | ||
| + | | || 20 mM || MgSO4 | ||
| + | |-align="center" | ||
| + | | || 50 mM || NaCl | ||
| + | |-align="center" | ||
| + | | || 0.01 % || gelatine | ||
| + | |-style="height:20px" | ||
| + | | || || | ||
| + | |-align="center" | ||
| + | | Tethering buffer, pH 7.0 || 10 mM || potassium phosphate | ||
| + | |-align="center" | ||
| + | | || || (K2HPO4 : KH2PO4 = 1 :1 ) | ||
| + | |-align="center" | ||
| + | | || 100 µM || EDTA | ||
| + | |-align="center" | ||
| + | | || 1 µM || L-Methionine | ||
| + | |-align="center" | ||
| + | | || 10 mM || lactic acid | ||
| + | |-style="height:20px" | ||
| + | | || || | ||
| + | |-align="center" | ||
| + | | M63 salt || 3 g/l || KH2PO4 | ||
| + | |-align="center" | ||
| + | | || 9 g/l || K2HPO4 | ||
| + | |-align="center" | ||
| + | | || 4 g/l || (NH4)2SO4 | ||
| + | |-align="center" | ||
| + | | || 0.5 g/l || FeSO4 | ||
| + | |-style="height:20px" | ||
| + | | || || | ||
| + | |-align="center" | ||
| + | | Amino acid mix || 5 g/l || L-threonine | ||
| + | |-align="center" | ||
| + | | || 5 g/l || L-histidin | ||
| + | |-align="center" | ||
| + | | || 5 g/l || L-leucine | ||
| + | |-align="center" | ||
| + | | || 5 g/l || L-methionine | ||
| + | |-style="height:20px" | ||
| + | | || || | ||
| + | |-align="center" | ||
| + | | M9 salts || 64 g/l || Na2HPO4 x 7H2O | ||
| + | |-align="center" | ||
| + | | || 15 g/l || KH2PO4 | ||
| + | |-align="center" | ||
| + | | || 2.5 g/l || NaCl | ||
| + | |-align="center" | ||
| + | | || 5 g/l || NH4Cl | ||
| + | |- | ||
| + | |} | ||
Revision as of 13:17, 28 October 2008
| SM | 50 mM | Tris, pH 7.5 |
| 20 mM | MgSO4 | |
| 50 mM | NaCl | |
| 0.01 % | gelatine | |
| Tethering buffer, pH 7.0 | 10 mM | potassium phosphate |
| (K2HPO4 : KH2PO4 = 1 :1 ) | ||
| 100 µM | EDTA | |
| 1 µM | L-Methionine | |
| 10 mM | lactic acid | |
| M63 salt | 3 g/l | KH2PO4 |
| 9 g/l | K2HPO4 | |
| 4 g/l | (NH4)2SO4 | |
| 0.5 g/l | FeSO4 | |
| Amino acid mix | 5 g/l | L-threonine |
| 5 g/l | L-histidin | |
| 5 g/l | L-leucine | |
| 5 g/l | L-methionine | |
| M9 salts | 64 g/l | Na2HPO4 x 7H2O |
| 15 g/l | KH2PO4 | |
| 2.5 g/l | NaCl | |
| 5 g/l | NH4Cl |
