Team:Heidelberg/Notebook/material

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Revision as of 13:37, 28 October 2008

Material

Buffers & Solution


SM	50 mM	Tris, pH 7.5
	20 mM	MgSO4
	50 mM	NaCl
	0.01 %	gelatine

Tethering buffer, pH 7.0	10 mM	potassium phosphate
		(K2HPO4 : KH2PO4 = 1 :1 )
	100 µM	EDTA
	1 µM	L-Methionine
	10 mM	lactic acid

M63 salt	3 g/l	KH2PO4
	9 g/l	K2HPO4
	4 g/l	(NH4)2SO4
	0.5 g/l	FeSO4

Amino acid mix	5 g/l	L-threonine
	5 g/l	L-histidin
	5 g/l	L-leucine
	5 g/l	L-methionine

M9 salts	64 g/l	Na2HPO4 x 7H2O
	15 g/l	KH2PO4
	2.5 g/l	NaCl
	5 g/l	NH4Cl

@@ Line 462: / Line 462: @@
 </body>
 </html>
+==Material==
+===Buffers & Solution===
 {| class="wikitable"
-|- bgcolor=grey; style="height:20px"
+|- bgcolor=grey
-! width=200px |  || width=250px |  || width=250px |
+! height=20px, width=200px |  || width=250px |  || width=250px |
 |-align="center"
-| SM || 50 mM || Tris, pH 7.5
+| style="font-weight:bold;" |SM || 50 mM || Tris, pH 7.5
 |-align="center"
-|  || 20 mM || MgSO4
+| style="font-weight:bold;" | || 20 mM || MgSO4
 |-align="center"
-|  || 50 mM || NaCl
+| style="font-weight:bold;" | || 50 mM || NaCl
 |-align="center"
-|  || 0.01 % || gelatine
+| style="font-weight:bold;" | || 0.01 % || gelatine
 |-style="height:20px"
 |  ||  ||
 |-align="center"
-| Tethering buffer, pH 7.0 || 10 mM  || potassium phosphate
+| style="font-weight:bold;" |Tethering buffer, pH 7.0 || 10 mM  || potassium phosphate
 |-align="center"
-|   ||  || (K2HPO4 : KH2PO4 = 1 :1 )
+| style="font-weight:bold;" |  ||  || (K2HPO4 : KH2PO4 = 1 :1 )
 |-align="center"
-|  || 100 µM || EDTA
+| style="font-weight:bold;" | || 100 µM || EDTA
 |-align="center"
-|  || 1 µM || L-Methionine
+| style="font-weight:bold;" | || 1 µM || L-Methionine
 |-align="center"
-|  || 10 mM || lactic acid
+| style="font-weight:bold;" | || 10 mM || lactic acid
 |-style="height:20px"
 |  ||  ||
 |-align="center"
-| M63 salt || 3 g/l || KH2PO4
+| style="font-weight:bold;" |M63 salt || 3 g/l || KH2PO4
 |-align="center"
-|  || 9 g/l || K2HPO4
+| style="font-weight:bold;" | || 9 g/l || K2HPO4
 |-align="center"
-|  || 4 g/l || (NH4)2SO4
+| style="font-weight:bold;" | || 4 g/l || (NH4)2SO4
 |-align="center"
-|  || 0.5 g/l || FeSO4
+| style="font-weight:bold;" | || 0.5 g/l || FeSO4
 |-style="height:20px"
 |  ||  ||
 |-align="center"
-| Amino acid mix || 5 g/l || L-threonine
+| style="font-weight:bold;" |Amino acid mix || 5 g/l || L-threonine
 |-align="center"
-|  || 5 g/l || L-histidin
+| style="font-weight:bold;" | || 5 g/l || L-histidin
 |-align="center"
-|  || 5 g/l || L-leucine
+| style="font-weight:bold;" | || 5 g/l || L-leucine
 |-align="center"
-|  || 5 g/l || L-methionine
+| style="font-weight:bold;" | || 5 g/l || L-methionine
 |-style="height:20px"
 |  ||  ||
 |-align="center"
-| M9 salts || 64 g/l || Na2HPO4 x 7H2O
+| style="font-weight:bold;" |M9 salts || 64 g/l || Na2HPO4 x 7H2O
 |-align="center"
-|  || 15 g/l || KH2PO4
+| style="font-weight:bold;" | || 15 g/l || KH2PO4
 |-align="center"
-|  || 2.5 g/l || NaCl
+| style="font-weight:bold;" | || 2.5 g/l || NaCl
 |-align="center"
-|  || 5 g/l || NH4Cl
+| style="font-weight:bold;" | || 5 g/l || NH4Cl
 |-
 |}