"
Team:KULeuven/Freezer
From 2008.igem.org
(Difference between revisions)
(→Parts stored in the fridge in the lab (4°C)) |
(→Parts stored in the fridge in the lab (4°C)) |
||
| Line 95: | Line 95: | ||
|width=90| '''Date''' | |width=90| '''Date''' | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_J23100 J23100]||''Eco''R I||''Spe'' I||Insert||52||38.1||2.6|| ||July 24 | + | |[http://partsregistry.org/Part:BBa_J23100 J23100]||''Eco''R I||''Spe'' I||Insert||52||38.1||2.6||1||July 24 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_C0040 C0040]|| ''Eco''R I||''Spe'' I||Insert||677||31.4||7.1|| ||July 24 | + | |[http://partsregistry.org/Part:BBa_C0040 C0040]|| ''Eco''R I||''Spe'' I||Insert||677||31.4||7.1||2||July 24 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_R0084 R0084]|| ''Eco''R I||''Spe'' I||Insert||125||33.4||3.8|| ||July 24 | + | |[http://partsregistry.org/Part:BBa_R0084 R0084]|| ''Eco''R I||''Spe'' I||Insert||125||33.4||3.8||3||July 24 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_E0022 E0022]|| ''Eco''R I||''Spe'' I||Insert||779||32.5||9.5|| ||July 24 | + | |[http://partsregistry.org/Part:BBa_E0022 E0022]|| ''Eco''R I||''Spe'' I||Insert||779||32.5||9.5||4||July 24 |
| + | |- style="background: #E0FFFF; text-align: center;" | ||
| + | |[http://partsregistry.org/Part:BBa_J23109 J23109]|| ''Eco''R I||''Spe'' I||Insert||52||28||4.2||5||July 24 | ||
| + | |- style="background: #E0FFFF; text-align: center;" | ||
| + | |[http://partsregistry.org/Part:BBa_R0084 R0084]|| ''Eco''R I||''Spe'' I||Insert||125||28||6.1||6||July 24 | ||
| + | |- style="background: #E0FFFF; text-align: center;" | ||
| + | |[http://partsregistry.org/Part:BBa_I712074 I712074]|| ''Eco''R I||''Spe'' I||Insert||62||28||3.4||7||July 24 | ||
|} | |} | ||
== Glycerol stock of cells (-80°C) == | == Glycerol stock of cells (-80°C) == | ||
Revision as of 12:04, 30 July 2008
Result:
To have a dropdown for your own team copy over all of the content below. It might be needed to adjust a thing or two. Comments have been added throughout the code what must be changed.
Inspirational websites:
- Most basic dropdown menu, with no submenu's
- The javascript library used to add effects and optimise the dropdown
- How to center a ul list effectively
The dropdown has been created and developed by the KULeuven team.
<html>
<style type="text/css">
#content {z-index:4;}
#ddwrapper * {z-index:8 !important;}
div#ddwrapper {
margin:0;
padding:0;
height:28px;
width:945px; /* change to adjust imperfections in width */
}
div#ddnav {
margin:0 auto; /* needed to center the dropdown */
padding:0;
top:5px;
/* width: 965px */
visibility:hidden; /* dropdown is hidden until properly initalised by javascript */
}
div#ddtoggle {
margin:0;
position:fixed;
right:2px;
top:15px;
height:10px;
width:10px;
z-index:100;
}
#ddnav ul {
display:table-row; /* works only for firefox, later adjusted by javascript for IE */
margin:0 auto;
padding:0;
}
#ddnav ul li {
display:table-cell; /* works only for firefox, later adjusted by javascript for IE */
list-style:none;
margin:0;
padding:0 !important;
border-right:1px solid #FFF; /* creates illusion of spacing between tabs */
}
#ddnav ul li:last-child{border-right:none;}
#ddnav a{
display:block;
margin:0;
padding:4px 14px; /* play with dimensions for size of tabs */
background-color:#075A90; /* background color for tabs */
color:#FFF !important; /* font color for text in tabs */
text-align:center; /* aligning for text in tabs */
text-decoration:none !important;
font:bold 10pt Trebuchet MS; /* font properties for text in tabs */
outline:0;
}
#ddnav ul li a:hover {background-color:#99CCFF;}/* background color for tabs on mouseover */
#ddnav li a:active {outline:none;} /* remove standard dotted border for links when clicked (IE) */
#ddnav li a:focus {-moz-outline-style:none;} /* remove standard dotted border for links when clicked (FF) */
#ddnav div {
display:none;
position:absolute;
width:9em;
background-color:#000; /* bug solution, do not change ! */
border:1px solid #5970B2; /* border color for dropdown menus */
opacity:0.9; /* transparancy of the dropdown menus (FF) */
filter:alpha(opacity=90); /* transparancy of the dropdown menus (IE) */
}
#ddnav div a {
display:block;
padding:5px 10px; /* play with dimensions of block element in dropdown menus */
position:relative;
font:normal 8pt arial; /* font properties for text in dropdown menus */
text-align:left; /* aligning of text in dropdown menus */
cursor:pointer;
}
#ddnav div a:hover, #ddnav span a:hover {color:#000 !important;} /* text color on mouseover */
#ddnav span div {
position:relative;
border:none;
border-bottom:2px groove #5970B2; /* separator for submenus, groove does not work in FF */
opacity:1.0; /* avoid stacking transparancy for submenus (FF) */
filter:alpha(opacity=100); /* avoid stacking transparancy for submenus (IE) */
}
/* may want to upload the following pictures to a new location */
.expand {background: url('https://static.igem.org/mediawiki/2008/e/ef/Icon-expand.png') no-repeat 95% 50%;}
.collapse {background: url('https://static.igem.org/mediawiki/2008/c/cd/Icon-collapse.png') no-repeat 95% 50%;}
.docked {background: #99ccff url("https://static.igem.org/mediawiki/2008/6/62/Ddnavundock.png") no-repeat 50% 50%;}
.undocked {background: #99ccff url("https://static.igem.org/mediawiki/2008/e/e4/Ddnavdock.png") no-repeat 50% 50%;}
</style>
<!-- IMPORTANT: save following script under a personalized webspace or download the library at www.jquery.com -->
<script type="text/javascript" src="http://student.kuleuven.be/~s0173901/wiki/js/jquery.js"></script>
<script type="text/javascript">
function ddnav() {
$('#ddnav ul li').hover(
function () {
$(this).find('div:first').css('display','block');},
function () {
$(this).find('div:first').css('display','none');}
);
}
function ddnavsub() {
$('#ddnav span > a').toggle(
function () {
$(this).removeClass("#ddnav expand").addClass("#ddnav collapse");
$(this).parent().find('div:first').slideDown('fast');
$(this).hover(
function (){$(this).css('background-color','#99AAFF');},
function (){$(this).css('background-color','#99AAFF');});},
function () {
$(this).removeClass("#ddnav collapse").addClass("#ddnav expand");
$(this).parent().find('div:first').css('display','none');
$(this).hover(
function (){$(this).css('background-color','#99CCFF');},
function (){$(this).css('background-color','#075A90');});}
).addClass("#ddnav expand");
}
/* If you wish to omit the docking feature, remove following function ddtoggle() */
function ddtoggle() {
$('#ddtoggle').toggle(
function () {
$(this).removeClass('undocked').addClass('docked');
$('#ddnav').css('position','fixed');},
function () {
$(this).removeClass('docked').addClass('undocked');
$('#ddnav').css('position','static');}
);
}
function ddalign() {
var _windowWidth = $(window).width();
var _leftOffset = (_windowWidth - 965)/2;
$('div#ddnav').css('left',_leftOffset);
}
function ddmsie() {
$('#ddnav a').hover(
function () {$(this).css('background-color','#99ccff');},
function () {$(this).css('background-color','#075a90');}
);
/* toggle doesn't work yet */
$('#ddtoggle').css('display','none');
$('#ddnav ul').css('display','inline-block');
$('#ddnav ul li').css('display','inline');
$('#ddnav ul li').css('position','relative');
$('#ddnav ul li>a').css('display','inline-block');
$('#ddnav ul li>a').css('margin-right','-4px');
$('#ddnav div').css('left','0');
$('#ddnav div').css('top','100%');
$('#ddnav span div').css('top','0');
}
function ddmozilla() {
ddtoggle();
$(window).bind('resize', function() {ddalign();});
}
$(function () {
ddnav();
ddnavsub();
if(jQuery.browser.msie) ddmsie();
if(jQuery.browser.mozilla) ddmozilla();
$('#ddnav').css('visibility','visible');
});
</script>
<!-- If you wish to omit the docking feature omit following line (div with id ddtoggle) -->
<div id="ddtoggle" class="undocked"></div>
<div id="ddwrapper">
<!-- Here the actual links are defined, simply replace with your own links in the appropriate sections -->
<div id="ddnav" align="center">
<ul>
<li>
<a href="https://2008.igem.org/Team:KULeuven">Home</a>
</li>
<li>
<a>The Team</a>
<div>
<a href="https://2008.igem.org/Team:KULeuven/Team/LabsandGroups">Research Labs and Groups</a>
<a href="https://2008.igem.org/Team:KULeuven/Team/Students">Students</a>
<a href="https://2008.igem.org/Team:KULeuven/Team/Instructors">Instructors</a>
<a href="https://2008.igem.org/Team:KULeuven/Team/Advisors">Advisors</a>
<a href="https://2008.igem.org/Team:KULeuven/Team/Pictures">Pictures</a>
</div>
</li>
<li>
<a>The Project</a>
<div>
<a href="https://2008.igem.org/Team:KULeuven/Project">Summary</a>
<span>
<a>Components</a>
<div>
<a href="https://2008.igem.org/Team:KULeuven/Project/Input">Input</a>
<a href="https://2008.igem.org/Team:KULeuven/Project/Output">Output</a>
<a href="https://2008.igem.org/Team:KULeuven/Project/Filter">Filter</a>
<a href="https://2008.igem.org/Team:KULeuven/Project/Inverter">Invertimer</a>
<a href="https://2008.igem.org/Team:KULeuven/Project/Reset">Reset</a>
<a href="https://2008.igem.org/Team:KULeuven/Project/CellDeath">Cell Death</a>
<a href="https://2008.igem.org/Team:KULeuven/Project/Memory">Memory</a>
</div>
</span>
<a href="https://2008.igem.org/Team:KULeuven/Evaluation">End Evaluation</a>
<a href="https://2008.igem.org/Team:KULeuven/Literature">Literature</a>
<a href="https://2008.igem.org/Team:KULeuven/Brainstorm">Brainstorm</a>
</div>
</li>
<li>
<a>Ethics</a>
<div>
</div>
</li>
<li>
<a>Submitted Parts</a>
<div>
<a href="https://2008.igem.org/Team:KULeuven/Parts">Listing</a>
<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=KULeuven">Sandbox</a>
</div>
</li>
<li>
<a>Modeling</a>
<div>
<a href="https://2008.igem.org/Team:KULeuven/Model/Overview">Overview</a>
<a href="https://2008.igem.org/Team:KULeuven/Model/KineticConstants">Kinetic Constants</a>
<span>
<a>Components</a>
<div>
<a href="https://2008.igem.org/Team:KULeuven/Model/Output">Output</a>
<a href="https://2008.igem.org/Team:KULeuven/Model/Filter">Filter</a>
<a href="https://2008.igem.org/Team:KULeuven/Model/Inverter">Invertimer</a>
<a href="https://2008.igem.org/Team:KULeuven/Model/Reset">Reset</a>
<a href="https://2008.igem.org/Team:KULeuven/Model/CellDeath">Cell Death</a>
<a href="https://2008.igem.org/Team:KULeuven/Model/Memory">Memory</a>
</div>
</span>
<a href="https://2008.igem.org/Team:KULeuven/Model/FullModel">Full Model</a>
<a href="https://2008.igem.org/Team:KULeuven/Model/Sensitivity">Sensitivity Analysis</a>
<a href="https://2008.igem.org/Team:KULeuven/Model/MultiCell">Multi-cell Model</a>
<a href="https://2008.igem.org/Team:KULeuven/Model/Diffusion">Diffusion</a>
</div>
</li>
<li>
<a>Data Analysis</a>
<div>
<a href="https://2008.igem.org/Team:KULeuven/Data/Overview">Overview</a>
<span>
<a>New Parts</a>
<div>
<a href="https://2008.igem.org/Team:KULeuven/Data/GFP">GFP (LVA-tag)</a>
<a href="https://2008.igem.org/Team:KULeuven/Data/T7">T7 (UmuD-tag)</a>
<a href="https://2008.igem.org/Team:KULeuven/Data/Antisense">Antisense LuxI</a>
<a href="https://2008.igem.org/Team:KULeuven/Data/ccdB">Celldeath (ccdB)</a>
<a href="https://2008.igem.org/Team:KULeuven/Data/HybridProm">Hybrid Promotor</a>
</div>
</span>
<span>
<a>Components</a>
<div>
<a href="https://2008.igem.org/Team:KULeuven/Data/Input">Input</a>
<a href="https://2008.igem.org/Team:KULeuven/Data/Output">Output</a>
<a href="https://2008.igem.org/Team:KULeuven/Data/Filter">Filter</a>
<a href="https://2008.igem.org/Team:KULeuven/Data/Inverter">Invertimer</a>
<a href="https://2008.igem.org/Team:KULeuven/Data/Reset">Reset</a>
<a href="https://2008.igem.org/Team:KULeuven/Data/CellDeath">Cell Death</a>
<a href="https://2008.igem.org/Team:KULeuven/Data/Memory">Memory</a>
</div>
</span>
<a href="https://2008.igem.org/Team:KULeuven/Data/FullModel">Full Model</a>
</div>
</li>
<li>
<a>Software</a>
<div>
<a href="https://2008.igem.org/Team:KULeuven/Software/MultiCell">Multi-cell Toolbox</a>
<a href="https://2008.igem.org/Team:KULeuven/Software/Simbiology2LaTeX">Simbiology2LaTeX Toolbox</a>
</div>
</li>
<li>
<a>Notebook</a>
<div>
<a href="https://2008.igem.org/Team:KULeuven/Meeting_Calendar">Calendar</a>
<a href="https://2008.igem.org/Team:KULeuven/SummerHolidays">Summer Holidays</a>
<span>
<a>Reports</a>
<div>
<a href="https://2008.igem.org/Team:KULeuven/Meeting Reports">Daily</a>
<a href="https://2008.igem.org/Team:KULeuven/Weekly Meetings">Weekly</a>
</div>
</span>
<span>
<a>Lab Data</a>
<div>
<a href="https://2008.igem.org/Team:KULeuven/Freezer">Freezer</a>
<a href="https://2008.igem.org/Team:KULeuven/Primers">Primers</a>
<a href="https://2008.igem.org/Team:KULeuven/Ligation">Ligation</a>
</div>
</span>
<a href="https://2008.igem.org/Team:KULeuven/Tools">Tools</a>
<a href="https://2008.igem.org/Team:KULeuven/Press">Press</a>
<a href="https://2008.igem.org/Team:KULeuven/Guestbook">Guestbook</a>
</div>
</li>
</ul>
</div>
</div>
</html>
Parts stored in the fridge in the lab (4°C)
| Plasmids: MiniPrep Products | ||||||
|---|---|---|---|---|---|---|
| Part | Plasmid | Description | µl left | ng/µl | Box | Date |
| M30109 | pSB1AC3 | Light-sensor | +/- 40 | 50.4 | 1 | July 23 |
| M30109 | pSB1AC3 | Light-sensor | +/- 30 | 54.3 | 2 | July 24 |
| J23100 | BBa_J61002 (Ap) | Constitutive promoter | +/- 60 | 345.0 | 3 | July 24 |
| J23100 | BBa_J61002 (Ap) | Constitutive promoter | +/- 30 | 176.1 | 4 | July 24 |
| B0034 | pSB1A2 | RBS (efficiency 1) | +/- 20 | 73.8 | 5 | July 24 |
| B0034 | pSB1A2 | RBS (efficiency 1) | +/- 30 | 95.1 | 6 | July 24 |
| B0034 | pSB1A2 | RBS (efficiency 1) | +/- 40 | 104.0 | 7 | July 29 |
| C0040 | pSB1A2 | TetR repressor | +/- 25 | 177.1 | 8 | July 24 |
| C0040 | pSB1A2 | TetR repressor | +/- 30 | 161.7 | 9 | July 24 |
| B0015 | pSB1AK3 | Terminator | +/- 40 | 63.0 | 10 | July 23 |
| B0015 | pSB1AK3 | Terminator | +/- 10 | 112.3 | 11 | July 24 |
| B0015 | pSB1AK3 | Terminator | +/- 20 | 130.3 | 12 | July 24 |
| B0015 | pSB1AK3 | Terminator | +/- 45 | 303.1 | 13 | July 29 |
| B0015 | pSB1AK3 | Terminator | +/- 30 | 213.6 | 14 | July 29 |
| R0084 | pSB1A2 | OmpF promoter | +/- 40 | 32.0 | 15 | July 23 |
| R0084 | pSB1A2 | OmpF promoter | +/- 30 | 60.9 | 16 | July 24 |
| B0032 | pSB1A2 | RBS (efficiency 0.3) | +/- 40 | 31.2 | 17 | July 23 |
| B0032 | pSB1A2 | RBS (efficiency 0.3) | +/- 10 | 114.8 | 18 | July 24 |
| E0022 | pSB1A2 | eCFP | +/- 40 | 84.2 | 19 | July 23 |
| E0022 | pSB1A2 | eCFP | +/- 40 | 86.4 | 20 | July 24 |
| C0012 | pSB1A2 | LacI | +/- 30 | 133.1 | 21 | July 29 |
| J23032 | BBa_J23006(Ap) | 3d lock | +/- 40 | 117.4 | 23 | July 29 |
| J23032 | BBa_J23006(Ap) | 3d lock | +/- 39.5 | 144.8 | 24 | July 29 |
| J23032 | BBa_J23006(Ap) | 3d lock | +/- 48 | 129.1 | 28 | July 29 |
| J23022 | BBa_J23006(Ap) | 3d key+ terminator | +/- 40 | 160.7 | 25 | July 29 |
| J23022 | BBa_J23006(Ap) | 3d key+ terminator | +/- 48 | 308.3 | 29 | July 29 |
| B0015 | BBa_J52017(Ap,Km) | Terminator | +/- 48 | 311.0 | 31 | July 29 |
| J23109 | BBa_J61002(Ap) | Constitutive Promotor | +/- 46 | 375.1 | 26 | July 29 |
| J23109 | BBa_J61002(Ap) | constitutive Pr | +/- 48 | 404 | 30 | July 29 |
| I712074 | BBa_J52017(Ap,Km) | T7 Promotor | +/- 42.5 | 208.2 | 27 | July 29 |
| F1610 | pSB1AK3 | RBS + LuxI + Terminator | +/- 40 | 104.0 | 22 | July 29 |
| Restriction Digests | ||||||||
|---|---|---|---|---|---|---|---|---|
| Part | Enzyme 1 | Enzyme 2 | vector/insert | length (bp) | µl left | ng/µl | Box | Date |
| J23100 | EcoR I | Spe I | Insert | 52 | 38.1 | 2.6 | 1 | July 24 |
| C0040 | EcoR I | Spe I | Insert | 677 | 31.4 | 7.1 | 2 | July 24 |
| R0084 | EcoR I | Spe I | Insert | 125 | 33.4 | 3.8 | 3 | July 24 |
| E0022 | EcoR I | Spe I | Insert | 779 | 32.5 | 9.5 | 4 | July 24 |
| J23109 | EcoR I | Spe I | Insert | 52 | 28 | 4.2 | 5 | July 24 |
| R0084 | EcoR I | Spe I | Insert | 125 | 28 | 6.1 | 6 | July 24 |
| I712074 | EcoR I | Spe I | Insert | 62 | 28 | 3.4 | 7 | July 24 |
