Team:LCG-UNAM-Mexico/Parts

From 2008.igem.org

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   &nbsp;<br /> </td>
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     <td colspan="4" valign="top"><div align="center"><img src="https://static.igem.org/mediawiki/2008/7/70/Head_spacer.gif" alt="" width="305" height="1" border="0" /><br />
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        <a href="#regparts"><img src="https://static.igem.org/mediawiki/2008/1/1f/Regparts1.jpg" alt="Regulatory parts" width="190" height="31" border="0"></a>    &nbsp;<a href="#codparts"><img src="https://static.igem.org/mediawiki/2008/2/25/Regparts2.jpg" alt="Coding parts" width="190" height="31" border="0"></a><a href="#primers"><img src="https://static.igem.org/mediawiki/2008/8/8b/Regparts3.jpg" alt="Primers" width="190" height="31" border="0"></a><a href="#devices"><img src="https://static.igem.org/mediawiki/2008/d/d9/Regparts4.jpg" alt="Devices" width="190" height="31" border="0"></a><br>
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        </div>
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      <table border="0" cellspacing="0" cellpadding="2" width="590">
           <tr>
           <tr>
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           <td colspan="4" class="pageName"><strong>Parts</strong></td>
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           <td colspan="4" class="pageName"><div align="center">Parts Submitted to the Registry </div></td>
         </tr>
         </tr>
        
        
  <tr>
  <tr>
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           <td colspan="4" class="pageName">Constructed Parts</td>
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           <td colspan="4" class="pageName"><div align="center"><br>
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              <span class="style1">Constructed Parts</span></div></td>
         </tr>
         </tr>
<tr>
<tr>
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   <td colspan="4" class="bodyText">The following are the bioparts that we are bringing into play for our project:</td>
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   <td colspan="4" class="bodyText">The following are the bioparts that we designed for our project:</td>
</tr>
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<tr>
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</tr>
</tr>
<tr>
<tr>
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   <td colspan="3" class="calHeader">Regulatory Parts</td>
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   <td colspan="3" class="calHeader style2"><a name="regparts"></a>Regulatory Parts</td>
   <td width="337" class="calHeader"><img src="http://partsregistry.org/images/partbypart/icon_regulatory.png" align="left
   <td width="337" class="calHeader"><img src="http://partsregistry.org/images/partbypart/icon_regulatory.png" align="left
   "></td>
   "></td>
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<tr>
<tr>
   <td colspan="2" class="subHeader">Description:</td>
   <td colspan="2" class="subHeader">Description:</td>
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   <td colspan="2" class="bodyText">LacZ constitutive promoter, without including operators. </td>
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   <td colspan="2" class="bodyText">LacZ constitutive promoter, without operators. </td>
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<tr>
   <td colspan="2" class="subHeader" >Description:</td>
   <td colspan="2" class="subHeader" >Description:</td>
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   <td colspan="2" rowspan="2" class="bodyText">Constitutive weak promoter of lacZ which sequence was changed at position 29 (T-&gt;G) in order to make it weaker</td>
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   <td colspan="2" rowspan="2" class="bodyText"><div align="justify">Constitutive weak promoter of lacZ which sequence was changed at position 29 (T-&gt;G) in order to make it weaker</div></td>
</tr>
</tr>
<tr>
<tr>
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<tr>
<tr>
   <td colspan="2" class="subHeader" >Description:</td>
   <td colspan="2" class="subHeader" >Description:</td>
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   <td colspan="2" class="bodyText">Operator for RcnR(YohL) the negative regulator of niquel extrusion pump(RcnA) in <i>E.coli</i> </td>
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   <td colspan="2" class="bodyText">Operator for RcnR(YohL) the repressor of nickel extrusion pump(RcnA) in <i>E.coli</i> </td>
</tr>
</tr>
<tr>
<tr>
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</tr>
</tr>
<tr>
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   <td colspan="3" class="calHeader">Coding Parts</td>
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   <td colspan="3" class="calHeader style2"><a name="codparts"></a>Coding Parts</td>
   <td class="calHeader"><img src="http://partsregistry.org/images/partbypart/icon_coding.png" alt="" align="left
   <td class="calHeader"><img src="http://partsregistry.org/images/partbypart/icon_coding.png" alt="" align="left
   "></td>
   "></td>
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<tr>
<tr>
   <td colspan="2" class="subHeader">Designed by:</td>
   <td colspan="2" class="subHeader">Designed by:</td>
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   <td colspan="2" rowspan="2" class="bodyText">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad  Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur  Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</td>
+
   <td colspan="2" rowspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad  Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur  Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</div></td>
</tr>
</tr>
<tr>
<tr>
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</tr>
<tr>
<tr>
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   <td colspan="3" class="calHeader">Primers</td>
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   <td colspan="3" class="calHeader style2"><a name="primers"></a>Primers</td>
   <td class="calHeader"><img src="http://partsregistry.org/images/partbypart/icon_primer.png" alt="" align="left
   <td class="calHeader"><img src="http://partsregistry.org/images/partbypart/icon_primer.png" alt="" align="left
   "></td>
   "></td>
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<tr>
<tr>
   <td colspan="2" class="subHeader">Part Name:</td>
   <td colspan="2" class="subHeader">Part Name:</td>
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   <td colspan="2" class="bodyText"><strong>Upper primer for aiiA with normal lacZ promoter </strong></td>
+
   <td colspan="2" class="bodyText"><strong>Upper primer for <em>aiiA</em> with normal lacZ promoter </strong></td>
</tr>
</tr>
<tr>
<tr>
   <td colspan="2" class="subHeader" >Description:</td>
   <td colspan="2" class="subHeader" >Description:</td>
-
   <td colspan="2" rowspan="2" class="bodyText"><p>Upper primer used to add lacZ promoter  to  aiiA in the device  LuxR_cI_aiiA_Normal lacZ promoter </p></td>
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   <td colspan="2" rowspan="2" class="bodyText"><p align="justify">Upper primer used to add lacZ promoter  to  aiiA in the device  LuxR_cI_aiiA_Normal lacZ promoter </p></td>
</tr>
</tr>
<tr>
<tr>
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<tr>
<tr>
   <td colspan="2" class="subHeader">Part Name:</td>
   <td colspan="2" class="subHeader">Part Name:</td>
-
   <td colspan="2" class="bodyText"><strong>Upper primer for aiiA with mutated lacZ promoter  </strong></td>
+
   <td colspan="2" class="bodyText"><strong>Upper primer for <em>aiiA</em> with mutated lacZ promoter  </strong></td>
</tr>
</tr>
<tr>
<tr>
   <td colspan="2" class="subHeader" >Description:</td>
   <td colspan="2" class="subHeader" >Description:</td>
-
   <td colspan="2" rowspan="2" class="bodyText"><p>Upper primer used to add a mutated version of lacZ promoter  to  aiiA in the device  LuxR_cI_aiiA_Normal lacZ promoter  </p></td>
+
   <td colspan="2" rowspan="2" class="bodyText"><p align="justify">Upper primer used to add a mutated version of lacZ promoter  to  <em>aiiA</em> in the device  LuxR_cI_aiiA_Normal lacZ promoter  </p></td>
</tr>
</tr>
<tr>
<tr>
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<tr>
<tr>
   <td colspan="2" class="subHeader" >Description:</td>
   <td colspan="2" class="subHeader" >Description:</td>
-
   <td colspan="2" rowspan="2" class="bodyText"><p>Upper primer used to add cI promoter upstream of the RcnR operator in the device cI+AVGreg_RcnRop_RcnA() </p></td>
+
   <td colspan="2" rowspan="2" class="bodyText"><p align="justify">Upper primer used to add cI promoter upstream of the RcnR operator in the device cI promoter RcnR op RcnA </p></td>
</tr>
</tr>
<tr>
<tr>
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<tr>
<tr>
   <td colspan="2" class="subHeader" >Description:</td>
   <td colspan="2" class="subHeader" >Description:</td>
-
   <td colspan="2" class="bodyText"><p>Lower primer for RcnA (BBa_K119003). Includes just the end of the RcnA coding region.</p></td>
+
   <td colspan="2" class="bodyText"><p align="justify">Lower primer for RcnA (BBa_K119003). Includes only the end of the RcnA coding region.</p></td>
</tr>
</tr>
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</tr>
</tr>
<tr>
<tr>
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   <td colspan="3" class="calHeader">Devices</td>
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   <td colspan="3" class="calHeader style2"><a name="devices"></a>Devices</td>
   <td class="calHeader">&nbsp;</td>
   <td class="calHeader">&nbsp;</td>
</tr>
</tr>
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<tr>
<tr>
   <td colspan="2" class="subHeader" >Description:</td>
   <td colspan="2" class="subHeader" >Description:</td>
-
   <td colspan="2" rowspan="2" class="bodyText"><p>This device encodes the extrusion pump and uses two repressible  promoters in order to stop the niquel extrusion. </p></td>
+
   <td colspan="2" rowspan="2" class="bodyText"><p align="justify">This device encodes the extrusion pump and uses two repressible  promoters in order to stop the niquel extrusion. </p></td>
</tr>
</tr>
<tr>
<tr>
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<tr>
<tr>
   <td colspan="2" class="subHeader">Designed by:</td>
   <td colspan="2" class="subHeader">Designed by:</td>
-
   <td colspan="2" rowspan="2" class="bodyText">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad  Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur  Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</td>
+
   <td colspan="2" rowspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad  Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur  Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</div></td>
</tr>
</tr>
<tr>
<tr>
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<tr>
<tr>
   <td colspan="2" class="subHeader" >Description:</td>
   <td colspan="2" class="subHeader" >Description:</td>
-
   <td colspan="2" rowspan="2" class="bodyText"><p>This device encodes cI, the protein which controls RcnA. It also contains LuxR, which Induces the expression of cI and aiiA </p></td>
+
   <td colspan="2" rowspan="2" class="bodyText"><p align="justify">This device encodes CI, the protein which controls <em>rcnA</em> expression. It also contains <em>luxR</em>, which induces the expression of <em>cI</em> and <em>aiiA.</em></p></td>
</tr>
</tr>
<tr>
<tr>
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<tr>
<tr>
   <td colspan="2" class="subHeader">Designed by:</td>
   <td colspan="2" class="subHeader">Designed by:</td>
-
   <td colspan="2" class="bodyText">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo</td>
+
   <td colspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo</div></td>
</tr>
</tr>
<tr>
<tr>
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<tr>
<tr>
   <td colspan="2" class="subHeader" >Description:</td>
   <td colspan="2" class="subHeader" >Description:</td>
-
   <td colspan="2" rowspan="2" class="bodyText"><p>This device encodes cI, the protein which controls RcnA. It also contains LuxR, which Induces the expression of cI and aiiA </p></td>
+
   <td colspan="2" rowspan="2" class="bodyText"><p align="justify">This device encodes CI, the protein which controls <em>rcnA</em>. It also contains <em>luxR</em>, which induces the expression of <em>cI</em> and <em>aiiA</em> </p></td>
</tr>
</tr>
<tr>
<tr>
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<tr>
<tr>
   <td colspan="2" class="subHeader">Designed by:</td>
   <td colspan="2" class="subHeader">Designed by:</td>
-
   <td colspan="2" class="bodyText">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo</td>
+
   <td colspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo</div></td>
</tr>
</tr>
<tr>
<tr>
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   <td colspan="2" class="calHeader">&nbsp;</td>
   <td colspan="2" class="calHeader">&nbsp;</td>
   </tr>    </table>
   </tr>    </table>
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       <br />
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       <div align="center"><br />
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&nbsp;<br />
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        &nbsp;<a href="#regparts"><img src="https://static.igem.org/mediawiki/2008/1/1f/Regparts1.jpg" alt="Regulatory parts" width="190" height="31" border="0"></a> &nbsp;<a href="#codparts"><img src="https://static.igem.org/mediawiki/2008/2/25/Regparts2.jpg" alt="Coding parts" width="190" height="31" border="0"></a><a href="#primers"><img src="https://static.igem.org/mediawiki/2008/8/8b/Regparts3.jpg" alt="Primers" width="190" height="31" border="0"></a><a href="#devices"><img src="https://static.igem.org/mediawiki/2008/d/d9/Regparts4.jpg" alt="Devices" width="190" height="31" border="0"></a><br>
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        <br>
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        <img src="https://static.igem.org/mediawiki/2008/9/99/Ribbon435773498.gif" alt="ribbon" width="579" height="9" /><br />
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  &nbsp;<br />
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  <br />
 +
        </div>
     <td width="132">&nbsp;</td>
     <td width="132">&nbsp;</td>
   </tr>
   </tr>

Revision as of 18:43, 28 October 2008

LCG-UNAM-Mexico:Parts

Header image
iGEM 2008 TEAM
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Regulatory parts  Coding partsPrimersDevices
ribbon
 
Parts Submitted to the Registry

Constructed Parts
The following are the bioparts that we designed for our project:
   
Regulatory Parts
BioBrick Number:

BBa_K119000
Part Name: Constitutive weak promoter of lacZ
Description: LacZ constitutive promoter, without operators.
Length: 38 bp
Designed by: Miguel Angel Ramirez-Romero
References:  
   
BioBrick Number:

BBa_K119001
Part Name: Mutated LacZ promoter
Description:
Constitutive weak promoter of lacZ which sequence was changed at position 29 (T->G) in order to make it weaker
 
Length: 38 bp
Designed by: Miguel Angel Ramirez-Romero
References:  
   
BioBrick Number:

BBa_K119002
Part Name: RcnR operator (represses RcnA)
Description: Operator for RcnR(YohL) the repressor of nickel extrusion pump(RcnA) in E.coli
Length: 83 bp
Designed by: Libertad Pantoja
References:  
   
Coding Parts
BioBrick Number:

BBa_K119003
Part Name: RcnA (YohM)
Description: Nickel efflux pump from E.coli
Length: 833 bp
Designed by:
Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo
 
References:  
   
Primers
BioBrick Number:

BBa_K119004
Part Name: Upper primer for aiiA with normal lacZ promoter
Description:

Upper primer used to add lacZ promoter to aiiA in the device LuxR_cI_aiiA_Normal lacZ promoter

 
Length: 67 bp
Designed by: Martin del Castillo V.
References:  
   
BioBrick Number:

BBa_K119005
Part Name: Upper primer for aiiA with mutated lacZ promoter
Description:

Upper primer used to add a mutated version of lacZ promoter to aiiA in the device LuxR_cI_aiiA_Normal lacZ promoter

 
Length: 67 bp
Designed by: Martin del Castillo V.
References:  
   
BioBrick Number:

BBa_K119007
Part Name: Upper primer for RcnA
Description:

Upper primer used to add cI promoter upstream of the RcnR operator in the device cI promoter RcnR op RcnA

 
Length: 73 bp
Designed by: Libertad Pantoja
References:  
   
BioBrick Number:

BBa_K119008
Part Name: Lower primer for RcnA (BBa_K119003)
Description:

Lower primer for RcnA (BBa_K119003). Includes only the end of the RcnA coding region.
Length: 21 bp
Designed by: Libertad Pantoja
References:  
   
Devices  
BioBrick Number:

BBa_K119009
Part Name: cI promoter RcnR op RcnA
Description:

This device encodes the extrusion pump and uses two repressible promoters in order to stop the niquel extrusion.

 
Length: 969 bp
Designed by:
Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo
 
References:  
   
BioBrick Number:

BBa_K119010
Part Name: LuxR_cI_aiiA_Normal lacZ promoter
Description:

This device encodes CI, the protein which controls rcnA expression. It also contains luxR, which induces the expression of cI and aiiA.
 
Length: 2873 bp
Designed by:
Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo
References:  
   
BioBrick Number:

BBa_K119011
Part Name: LuxR_cI_aiiA_Mutated lacZ promoter
Description:

This device encodes CI, the protein which controls rcnA. It also contains luxR, which induces the expression of cI and aiiA

 
Length: 2873 bp
Designed by:
Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo
References:  
   
     

 Regulatory parts  Coding partsPrimersDevices

ribbon
 

 
             

Retrieved from "http://2008.igem.org/Team:LCG-UNAM-Mexico/Parts"