Team:LCG-UNAM-Mexico/Parts

From 2008.igem.org

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   <td colspan="2" rowspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad  Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur  Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</div></td>
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   <td colspan="2" rowspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad  Pantoja, B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur  Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</div></td>
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   <td colspan="2" rowspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad  Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur  Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</div></td>
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   <td colspan="2" rowspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad  Pantoja, B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur  Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</div></td>
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   <td colspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo</div></td>
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   <td colspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo</div></td>
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   <td colspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo</div></td>
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   <td colspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo</div></td>
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Latest revision as of 01:34, 26 November 2008

LCG-UNAM-Mexico:Parts

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iGEM 2008 TEAM
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Regulatory parts  Coding partsPrimersDevices
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Parts Submitted to the Registry

Constructed Parts
The following are the bioparts that we designed for our project:
   
Regulatory Parts
BioBrick Number:

BBa_K119000

Part Name: Constitutive weak promoter of lacZ
Description: LacZ constitutive promoter, without operators.
Length: 38 bp
Designed by: Miguel Angel Ramirez-Romero
References:  
   
BioBrick Number:

BBa_K119001

Part Name: Mutated LacZ promoter
Description:
Constitutive weak promoter of lacZ which sequence was changed at position 29 (T->G) in order to make it weaker
 
Length: 38 bp
Designed by: Miguel Angel Ramirez-Romero
References:  
   
BioBrick Number:

BBa_K119002

Part Name: RcnR operator (represses RcnA)
Description: Operator for RcnR(YohL) the repressor of nickel extrusion pump(RcnA) in E.coli
Length: 83 bp
Designed by: Libertad Pantoja
References:  
   
Coding Parts
BioBrick Number:

BBa_K119003

Part Name: RcnA (YohM)
Description: Nickel efflux pump from E.coli
Length: 833 bp
Designed by:
Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo
 
References:  
   
Primers
BioBrick Number:

BBa_K119004

Part Name: Upper primer for aiiA with normal lacZ promoter
Description:

Upper primer used to add lacZ promoter to aiiA in the device LuxR_cI_aiiA_Normal lacZ promoter

 
Length: 67 bp
Designed by: Martin del Castillo V.
References:  
   
BioBrick Number:

BBa_K119005

Part Name: Upper primer for aiiA with mutated lacZ promoter
Description:

Upper primer used to add a mutated version of lacZ promoter to aiiA in the device LuxR_cI_aiiA_Normal lacZ promoter

 
Length: 67 bp
Designed by: Martin del Castillo V.
References:  
   
BioBrick Number:

BBa_K119007

Part Name: Upper primer for RcnA
Description:

Upper primer used to add cI promoter upstream of the RcnR operator in the device cI promoter RcnR op RcnA

 
Length: 73 bp
Designed by: Libertad Pantoja
References:  
   
BioBrick Number:

BBa_K119008

Part Name: Lower primer for RcnA (BBa_K119003)
Description:

Lower primer for RcnA (BBa_K119003). Includes only the end of the RcnA coding region.

Length: 21 bp
Designed by: Libertad Pantoja
References:  
   
Devices  
BioBrick Number:

BBa_K119009

Part Name: cI promoter RcnR op RcnA
Description:

This device encodes the extrusion pump and uses two repressible promoters in order to stop the niquel extrusion.

 
Length: 969 bp
Designed by:
Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo
 
References:  
   
BioBrick Number:

BBa_K119010

Part Name: LuxR_cI_aiiA_Normal lacZ promoter
Description:

This device encodes CI, the protein which controls rcnA expression. It also contains luxR, which induces the expression of cI and aiiA.

 
Length: 2873 bp
Designed by:
Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo
References:  
   
BioBrick Number:

BBa_K119011

Part Name: LuxR_cI_aiiA_Mutated lacZ promoter
Description:

This device encodes CI, the protein which controls rcnA. It also contains luxR, which induces the expression of cI and aiiA

 
Length: 2873 bp
Designed by:
Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo
References:  
   
     

 Regulatory parts  Coding partsPrimersDevices

ribbon