Team:Montreal/Notebook/July

From 2008.igem.org

(Difference between revisions)
(July 1)
 
(One intermediate revision not shown)
Line 82: Line 82:
=== July 1 ===
=== July 1 ===
 +
*Maxi-prep of J-brick, assuming high copy number plasmid.
 +
 +
=== July 2 ===
 +
*Gel of J-brick maxi-prep, results indicate large plasmid at well over 13K. Assumed incorrect, to be repeated.
 +
 +
=== July 3 ===
 +
*Repeat gel, of J-brick maxi prep from July 1st, negative results. Gel from July 2nd assumed contamination from other lanes.
 +
 +
=== July 4 ===
 +
*Preparation of media.
 +
 +
=== July 7 ===
 +
*Seeding of J-brick for mini-prep, 4x5mL cultures with ampicillin.
 +
 +
=== July 8 ===
 +
*Mini-prep of J-brick cultures followed by gel giving negative results - no DNA seen, but ladder appears normal.
 +
 +
=== July 9 ===
 +
*

Latest revision as of 19:52, 11 August 2008

Home The Team The Project Parts Submitted to the Registry Modeling Notebook

July 2008
T W Th F Sa Su M
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 29
29 30


Contents

July 2008

July 1

  • Maxi-prep of J-brick, assuming high copy number plasmid.

July 2

  • Gel of J-brick maxi-prep, results indicate large plasmid at well over 13K. Assumed incorrect, to be repeated.

July 3

  • Repeat gel, of J-brick maxi prep from July 1st, negative results. Gel from July 2nd assumed contamination from other lanes.

July 4

  • Preparation of media.

July 7

  • Seeding of J-brick for mini-prep, 4x5mL cultures with ampicillin.

July 8

  • Mini-prep of J-brick cultures followed by gel giving negative results - no DNA seen, but ladder appears normal.

July 9