From 2008.igem.org

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 {{:Team:Newcastle University/Header}}
 {{:Team:Newcastle University/Template:UnderTheWetLab|page-title=[[Team:Newcastle University/Protocols|Protocols]]}}
+{{:Team:Newcastle University/WetlabSideBar}}
+<div id="maincol">
+==Protocols==
 [[Agarose Gel Electrophoresis]]
+[[Making Agar Plates]]
 [[Isolating Plasmid from Cells (Miniprep)]]
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 [[Restricting Plasmids (Double Restriction)]]
-[[Ligation]]
+[[Purifying DNA from an enzymatic reaction]]
+[[Cutting a Specific Band from Agarose Gel]]
+[[Purifying DNA from Gel Slices]]
+[[Ligating DNA]]
+[[Transforming into DH5α or TOP10 E. coli]]
+[[Making Frozen Glycerol Cell Stocks From Overnight Cultures]]
+[[Making Overnight Cultures from Frozen Glycerol Cell Stocks]]
-[[Transformation Following Ligation]]
+[[Making Overnight Cultures from Agar Colonies]]
-[[Making Frozen Glycerol Cell Stocks]]
+[[Transforming into Bacillus subtillis]]
-===Making Frozen Glycerol Cell Stocks===
+[[Inducing Bacillus subtilis with Subtilin]]
-* Pippette 2μl overnight culture into an Eppendorf tube.
+[[Preparing Bacillus subtilis for Microscopy]]
-* Add 0.5ml glycerol and vortex briefly to mix.
+</div>
-* Prepare a freeze box by decanting a small amount of liquid nitrogen into the box. (N.B: wear protective gloves and face mask.)
-* Place the tube in the box and keep at -80°C.