Team:Paris/Analysis/Construction2

From 2008.igem.org

Revision as of 21:13, 25 October 2008 by Felipe (Talk | contribs)

Model Construction


Description

  • We chose to use a chemostat. We want to impose to our model the fact that the rate of production has to be proportional to the existing population and to the amount of available resources.
  • We choose a logistic model.
  • To archieve synchronization we use QS. Explain the principle?

Kinetics

  • Hence a logistic model of the population, where c denotes the concentration of cells in the medium:


Population evolution 2.jpg


  • Then, we need to add the death term, and a dilution term cause by the renewal of the chemostat. Finally we get :


Population evolution full 2.jpg


where cmax is the carrying capacity for cell growth, and D renewal.jpg the dilution rate, d the death rate constant

  • Production of HSL:

eqHSL 1

  • Transport of HSL:

eqHSL 2

  • Activation of envZ depends of the concentration of HSL:

eqHSL 3


Parameters Search

manly from literature but also from S0 analysis.

Parameters
 
Chemostat Parameter Meaning Original Value Normalized Value Unit Source
figure / equations of Chemostat αcell Growth rate 0.0198 1 min-1 wet-lab
cmax Carrying capacity for cell growth 0.1 0.1 µm3 [3]
Drenewal Dilution rate 0.00198 0.1 min-1 wet-lab ([3])
d Death rate 0.0099 0.5 min-1 wet-lab
 
Quorum Sensing Parameter Meaning Original Value Normalized Value Unit Source
figure / equations of Quorum Sensing γHSL Degradation rate 0.0053 0.2690 min-1 wet-lab
γHSLext

Degradation rate 0.0106 0.5380 min-1 [6]
βHSL Production rate 0.3168 16 min-1
η Diffusion rate 10 505 min-1 [2]
nHSL Hill coefficient   4   [3]
θHSL Hill characteristic concentration for the second operator   0.5 c.u [3]