Generating a FIFO sequential order of expression

A clock that would only indicate one hour, would not be very useful. The same goes for our BacteriO'clock. It is necessary that we add several distinct events in each cycle. We want to use three genes activated successively.

The easiest way to control a sequential expression of gene

The washing of dirty plates traditionally follows a LIFO order : the last plate on the stack is the first one to be washed

The temporal order of expression that is the easiest to set up is the Last In First Out temporal order. A sequence of events that is traditionally a LIFO is the washing of dirty plates : the Last plate on the stack is the first one to be washed

In the FIFO you could start by saying we were looking for a FIFO and rather than designing it from scratch we decided to look in nature where we found and adopted the flagella which then inspired us further

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