Team:Paris/First page

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{{Paris/Menu}}
{{Paris/Menu}}
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<center><html><div style="color:#275D96; font-size:2em;">Grosse GROSSE chatte</div></html></center>
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<center><html><div style="color:#275D96; font-size:2em;">Grosse GROSSE GROOOOOSSSEEEEE Pomme de Terre !</div></html></center>
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= The BacterioClock =  
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= The BacteriO'Clock =  
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Were you dreaming about a new way of thinking everyday life objects ? Be sure that this will come true, under the form of our BacterioClock ! A simple tube which contains the modified bacteria we have created will give you the time, directly coming from living organisms.
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Have you ever dreamt of a new way of thinking everyday life objects ? Be sure, this will come true, under the form of our BacteriO'Clock ! A simple test tube which contains our modified bacteria will give you the time, directly from living organisms. The hours of the day are color-coded and oscillations ensure the repeated periodic behaviour.
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<embed  width="213" height="160"  src="http://biosynthetique.free.fr/videos/flv_player/flvplayer.swf?autostart=true&amp;repeat&amp;file=http://perso.strepsiade.org/~gvieira/out2.flv" quality="high" type="application/x-shockwave-flash"  allowfullscreen="true" align="right"/>
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[Video_de_ouf_!|center]
 
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To achieve this innovative project, we have decided to rely on a genetic structure that allows a specific sequence of lighting (Firt In - First Out behaviour) to occur. The models designed have shown that simply adding a negative feedback from a lighting gene to the first activator will not yield oscillations ! Therefore, we have proposed to induce a necessary delay, through a synchronization process among the population, as represented below :
 
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[[Image:Image_qui_dechire_touuuut|center]]
 
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DEGAGER
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We hereby wish to present our ambitious BacteriO'Clock!
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Synthetic biology is a new science, so we have to chance to set the base of synthetic biology. Hence our motto : '''Innovation'''! Therefore, we put a lot of efforts in proposing an original approach while building our system and analysing it.
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To achieve this incredible project, we relied on a well characterized genetic structure that allows a specific sequence of fluorescent proteins expression to occur in a First In - First Out order. This FIFO behaviour enables us to define a rich color encoding of day time. An additional negative feedback loop generates clock oscillations!  
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We shall present here our major results. Then we wished to show how we tried to meet iGEM goals : "Can simple biological systems be built from standard, interchangeable parts and operated in living cells? Or is biology just too complicated to be engineered in this way?".
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= Achievements =
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First of all, we intended to build a multipurpose system, that would be able to behave as a FIFO and oscillate.
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Up to now, we managed to build biologically a system that is to behave as a FIFO, and designed the oscillatory system. We also foresaw improvments that would enhance our system performances, among which introducing a synchronization application.
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'''Our biological results up to now'''
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''' Promote the open and transparent development of tools for engineering biology '''
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We have worked under the constraint that the research we carried would not content ourselves with proposing solutions for our system only. We are proud to propose two major approaches in that direction.
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* First of all, usually systems are designed biologically only. We stressed on the importance that a system behavior could be predicted with few data. Right after the overall system had been designed by the whole team, we built quickly a first model that would help us
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1.prédire les comportements avec un minimum de données! and then use biological data to understand par ex
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shéma model->lab->model donner exemple FIFO<br>
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2.akaike
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''' Enable the systematic engineering of biology '''
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1.biobriks<br>
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2.caractérisation Hugo
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3.conception de protocoles systématiques, trouver des infos qui ne soient pas propres a notre propre systeme
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volonté d'établir des protocoles, standardiser les processus ingénieriser/montrer que la bio n'est pas trop compliquée a ingénieriser
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''' Construct a society that can productively apply biological technology '''
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projet bioplastiques//productivité+ pour l'industrie
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= The team ! =
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multidisciplinaire donc différentes cultures enrichissantes! undergrad 20 personnes en alternances dou organisation
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= Links =
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Based on in-depth studies and experimentally measured parameters we develope models that show that this system is not likely to oscillate, and indicate ways to improve the initial design. In particular, this can be achieved by HSL production that enforces a necessary delay and provides an elegant cell synchronization mechanism :
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[[Image:Unimo.png|center|Simple visualization of our color coded BacteriO'Clock]]
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=sponsors=
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In a nutshell, the new trendy item which will make every biologist in your lab jealous will soon be available. Despise a long-cloning period all biobricks and other constructions are now available. Furthermore, we initiated an extensive characterization plan of these parts. If you manage to wait for the updates, you might even get the version which automatically react to daylight saving time ;)

Latest revision as of 01:20, 30 October 2008


Grosse GROSSE GROOOOOSSSEEEEE Pomme de Terre !


The BacteriO'Clock

Have you ever dreamt of a new way of thinking everyday life objects ? Be sure, this will come true, under the form of our BacteriO'Clock ! A simple test tube which contains our modified bacteria will give you the time, directly from living organisms. The hours of the day are color-coded and oscillations ensure the repeated periodic behaviour.

To achieve this incredible project, we relied on a well characterized genetic structure that allows a specific sequence of fluorescent proteins expression to occur in a First In - First Out order. This FIFO behaviour enables us to define a rich color encoding of day time. An additional negative feedback loop generates clock oscillations!

Based on in-depth studies and experimentally measured parameters we develope models that show that this system is not likely to oscillate, and indicate ways to improve the initial design. In particular, this can be achieved by HSL production that enforces a necessary delay and provides an elegant cell synchronization mechanism :

Simple visualization of our color coded BacteriO'Clock

In a nutshell, the new trendy item which will make every biologist in your lab jealous will soon be available. Despise a long-cloning period all biobricks and other constructions are now available. Furthermore, we initiated an extensive characterization plan of these parts. If you manage to wait for the updates, you might even get the version which automatically react to daylight saving time ;)