Team:Paris/Modeling/f2

From 2008.igem.org

(Difference between revisions)
Line 5: Line 5:
[[Image:f2.jpg|thumb|Specific Plasmid Characterisation for ƒ2]]
[[Image:f2.jpg|thumb|Specific Plasmid Characterisation for ƒ2]]
-
The experience would give us
+
According to the characterization plasmid (see right) and to our modeling, in the '''exponential phase of growth''', at the steady state, the experiment would give us
[[Image:f2expr.jpg|center]]
[[Image:f2expr.jpg|center]]
Line 15: Line 15:
<div style="text-align: center">
<div style="text-align: center">
-
{{Paris/Toggle|Table|Team:Paris/Modeling/More_f2_Table}}  
+
{{Paris/Toggle|Table of Values|Team:Paris/Modeling/More_f2_Table}}  
</div>
</div>
<div style="text-align: center">
<div style="text-align: center">
-
{{Paris/Toggle|Algorithm|Team:Paris/Modeling/More_f2_Algo}}  
+
{{Paris/Toggle|Algorithms|Team:Paris/Modeling/More_f2_Algo}}  
</div>
</div>

Revision as of 01:49, 30 October 2008

Method & Algorithm : ƒ2


Specific Plasmid Characterisation for ƒ2

According to the characterization plasmid (see right) and to our modeling, in the exponential phase of growth, at the steady state, the experiment would give us

F2expr.jpg

and at steady-state and in the exponential phase of growth, we expect :

Exprpbad.jpg


↓ Table of Values ↑


param signification unit value comments
(fluorescence) value of the observed fluorescence au need for 20 measures with well choosen [arab]i
conversion conversion ratio between
fluorescence and concentration
↓ gives ↓
nM.au-1 (1/79.429)
[GFP] GFP concentration at steady-state nM
γGFP dilution-degradation rate
of GFP(mut3b)
↓ gives ↓
min-1 0.0198 Only Dilution
Time Cell Disvision : 35 min.
ƒ2 activity of
pBad with RBS E0032
nM.min-1



param signification
corresponding parameters in the equations
unit value comments
βbad total transcription rate of
pBad with RBS E0032
not in the Core System
nM.min-1
(γ Kbad/const.expr(pBad)) activation constant of pBad
not in the Core System
nM
nbad complexation order of pBad
not in the Core System
no dimension The literature [?] gives nbad =
Kara complexation constant Arabinose><AraC
not in the Core System
nM The literature [?] gives Kara =
nara complexation order Arabinose><AraC
not in the Core System
no dimension The literature [?] gives nara =


↓ Algorithms ↑


find_ƒ2

function optimal_parameters = find_f2(X_data, Y_data, initial_parameters)
% gives the 'best parameters' involved in f2 by least-square optimisation
 
% X_data = vector of given values of a [arab]i (experimentally
% controled)
% Y_data = vector of experimentally measured values f2 corresponding of
% the X_data
% initial_parameters = values of the parameters proposed by the literature
%                       or simply guessed
%                    = [betabad, (Kbad -> (gamma.Kbad)/(const.expr(pBad))), nbad, Kara, nara]
 
     function output = expr_pBad(parameters, X_data)
         for k = 1:length(X_data)
                 output(k) = parameters(1) * ( hill( ...
                     (hill(X_data(k), parameters(4), parameters(5))), parameters(2), parameters(3)) );
         end
     end
 
options=optimset('LevenbergMarquardt','on','TolX',1e-10,'MaxFunEvals',1e10,'TolFun',1e-10,'MaxIter',1e4);
% options for the function lsqcurvefit
 
optimal_parameters = lsqcurvefit( @(parameters, X_data) expr_pBad(parameters, X_data), ...
     initial_parameters, X_data, Y_data, 1/10*initial_parameters, 10*initial_parameters, options );
% search for the fittest parameters, between 1/10 and 10 times the initial
% parameters
end

Inv_ƒ2

function quant_ara = Inv_f2(inducer_quantity)
% gives the quantity of [ara]i needed to get inducer_quantity of a protein
% throught a gene behind pBad
 
     function equa = F(x)
         equa = f2( x ) - inducer_quantity;
     end
 
options=optimset('LevenbergMarquardt','on','TolX',1e-10,'MaxFunEvals',1e10,'TolFun',1e-10,'MaxIter',1e4);
 
quant_ara = fsolve(F,1,options);
 
end

That will give us directly ƒ2([arab])


<Back - to "Implementation" |
<Back - to "Protocol Of Characterization" |