Team:Paris/Notebook/Freezer

From 2008.igem.org

(Difference between revisions)

Revision as of 18:43, 22 July 2008

PCR products

PCR products
Number	Name	Oligo F	Oligo R	Matrix	Length	Comments	Product Left ?	[]ng/µL	Date
									[[ ]]
									[[ ]]

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number	Name	Biobricks	Description	Products in use	Date
MP1	pJ23100 in J61002		Strong constitutive promoter in J61002		2007
MP2	pJ23107 in J61002		Medium constitutive promoter in J61002	MP2.2	2007
MP3	B0015 in pSB1AK3		Double terminator	MP3.3, MP3.4	2007
MP4	I0500 in pSB2K3		AraC pBAD		2007
MP5	B0030 in pSB1A2		Strong RBS (Efficiency = 0,6)		2007
MP6	E0422 in pSB1A2		ECFP (RBS+LVA+Term)	MP6'.1, MP6'.2	2007
MP8	E0840 in pSB1A2		gfp tri-part; strong rbs		2007

Plasmids: MiniPrep "in preparation"
Number	Name	Biobricks	Description	Products in use	Date
	B0034		Strongest RBS (Efficiency = 1)		2008
	C0040		tetR repressor		2008
	C0079		lasR activator with LVA Tag		2008
	C0179		lasR activator		2007
	E0030		YFP		2008
	E0040		GFP		2008
	E1010		mRFP		2008
	J23101		Strongest Constitutive promoter		2008
	J23109		Weak Constitutive promoter		2008
	R0040		tetR repressible promoter		2008
	R0079		promoter pLas (lasr & PAI regulated)		2008
	S03154		B0034(rbs) ->LasI		2008
	S03879		B0034(rbs) -> TetR		2008

Digestion Products

(Legend: Vector, Insert)

Digestion Products
Number	Product Name	Matrix Name	Enzyme 1	Enzyme 2	Size	Description	Product Left	[]ng/µL	Date
D1	<bbpart>pJ23100</bbpart>	<bbpart>pJ23100</bbpart>	Spe1	Pst1	2096	Vector with the J23100 promoter ready for insertion of a RBS/CDS insert	Ø (24/09/07)	34	July 17
D2	<bbpart>mRFP dblTer</bbpart>	<bbpart>pJ23100</bbpart>	Spe1	Pst1	887			27	July 17

@@ Line 59: / Line 59: @@
 |align="center"|[http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002]
 |align="center"|[[Image:Part_icon_regulatory.png]]
-|align="center"|Strong constitutive promoter in J61002 (clones 1 + 2)
+|align="center"|Strong constitutive promoter in J61002
 |
 |align="center"|2007
@@ Line 66: / Line 66: @@
 |align="center"|[http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002]
 |align="center"|[[Image:Part_icon_regulatory.png]]
-|align="center"|Medium constitutive promoter in J61002 (clones 1 + 2)
+|align="center"|Medium constitutive promoter in J61002
 |align="center"|MP2.2
 |align="center"|2007
@@ Line 73: / Line 73: @@
 |align="center"|[http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3]
 |align="center"|[[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-|align="center"|Double terminator (clones 1 + 2)
+|align="center"|Double terminator
 |align="center"|MP3.3, MP3.4
 |align="center"|2007
@@ Line 80: / Line 80: @@
 |align="center"|[http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3]
 |align="center"|[[Image:Part_icon_regulatory.png]]
-|align="center"|AraC pBAD (clones 1 + 2)
+|align="center"|AraC pBAD
 |
 |align="center"|2007
@@ Line 87: / Line 87: @@
 |align="center"|[http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]
 |align="center"|[[Image:Part_icon_rbs.png]]
-|align="center"|Strong RBS (clones 1 + 2)
+|align="center"|Strong RBS (Efficiency = 0,6)
 |
 |align="center"|2007
@@ Line 94: / Line 94: @@
 |align="center"|[http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]
 |align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
-|align="center"|ECFP (RBS+LVA+Term) (clones 1 + 2)
+|align="center"|ECFP (RBS+LVA+Term)
 |align="center"|MP6'.1, MP6'.2
 |align="center"|2007
@@ Line 101: / Line 101: @@
 |align="center"|[http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]
 |align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
-|align="center"|gfp tri-part; strong rbs (clones 1 + 2)
+|align="center"|gfp tri-part; strong rbs
 |
 |align="center"|2007
@@ Line 122: / Line 122: @@
 |align="center"|[http://partsregistry.org/Part:BBa_B0034 B0034]
 |align="center"|[[Image:Part_icon_rbs.png]]
-|align="center"|Strongest RBS
+|align="center"|Strongest RBS (Efficiency = 1)
 |
 |align="center"|2008