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Revision as of 18:43, 22 July 2008 by
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Project
Analysis
Construction
Characterization
Perspectives
PCR products
PCR products
Number
Name
Oligo F
Oligo R
Matrix
Length
Comments
Product Left ?
[]ng/µL
Date
[[ ]]
[[ ]]
Plasmids: MiniPrep Products
Plasmids: MiniPrep Products
Number
Name
Biobricks
Description
Products in use
Date
MP1
pJ23100
in
J61002
Strong constitutive promoter in J61002
2007
MP2
pJ23107
in
J61002
Medium constitutive promoter in J61002
MP2.2
2007
MP3
B0015
in
pSB1AK3
Double terminator
MP3.3, MP3.4
2007
MP4
I0500
in
pSB2K3
AraC pBAD
2007
MP5
B0030
in
pSB1A2
Strong RBS (Efficiency = 0,6)
2007
MP6
E0422
in
pSB1A2
ECFP (RBS+LVA+Term)
MP6'.1, MP6'.2
2007
MP8
E0840
in
pSB1A2
gfp tri-part; strong rbs
2007
Plasmids: MiniPrep "in preparation"
Number
Name
Biobricks
Description
Products in use
Date
B0034
Strongest RBS (Efficiency = 1)
2008
C0040
tetR repressor
2008
C0079
lasR activator with LVA Tag
2008
C0179
lasR activator
2007
E0030
YFP
2008
E0040
GFP
2008
E1010
mRFP
2008
J23101
Strongest Constitutive promoter
2008
J23109
Weak Constitutive promoter
2008
R0040
tetR repressible promoter
2008
R0079
promoter pLas (lasr & PAI regulated)
2008
S03154
B0034(rbs) ->LasI
2008
S03879
B0034(rbs) -> TetR
2008
Digestion Products
(Legend:
Vector
,
Insert
)
Digestion Products
Number
Product Name
Matrix Name
Enzyme 1
Enzyme 2
Size
Description
Product Left
[]ng/µL
Date
D1
<bbpart>pJ23100</bbpart>
<bbpart>pJ23100</bbpart>
Spe1
Pst1
2096
Vector with the J23100 promoter ready for insertion of a RBS/CDS insert
Ø (24/09/07)
34
July 17
D2
<bbpart>mRFP dblTer</bbpart>
<bbpart>pJ23100</bbpart>
Spe1
Pst1
887
27
July 17