Team:Paris/Project/Synchronization

From 2008.igem.org

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(→First Model)
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|-
|-
|align="center"|FlhDC
|align="center"|FlhDC
-
|align="center"|pTet - FlhDC - CFP
+
|align="center"|pTet - FlhDC
|align="center"|☣
|align="center"|☣
|-
|-
-
|align="center"|Reporter
+
|align="center"|AHL enzyme
-
|align="center"|pFliL - LasI - GFP
+
|align="center"|pFliL - LasI
|align="center"|☣
|align="center"|☣
|-
|-
-
|align="center"|Reporter
+
|align="center"|AHL Receptor
|align="center"|pConstitutive - LasR
|align="center"|pConstitutive - LasR
|align="center"|☣
|align="center"|☣
|-
|-
-
|align="center"|Reporter
+
|align="center"|FlhDC Inhibitor
|align="center"|pLas - TetR - RFP
|align="center"|pLas - TetR - RFP
|align="center"|☣
|align="center"|☣

Revision as of 19:38, 17 July 2008

Synchronization

First Model

Synchro1.jpg

Strain : double mutant ΔflhDC and ΔflgM

Designed plasmids

Generic Name Construction Advancement
FlhDC pTet - FlhDC ☣
AHL enzyme pFliL - LasI ☣
AHL Receptor pConstitutive - LasR ☣
FlhDC Inhibitor pLas - TetR - RFP ☣

Second Model

Synchro2.jpg


Strain : mutant ΔflgM (fliA and flhDC and their promotors are actually on the bacterial chromosome)

Designed plasmids

Generic Name Construction Advancement
Reporter pFliL - TetR - GFP ☣
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