Team:Rice University/STRATEGY

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(Metabolic Parts)
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[[Image:TAL.png|right|150px|thumb|TAL monomer complexed with ''p''-coumaric acid.  Active His is shown in black.]]
[[Image:TAL.png|right|150px|thumb|TAL monomer complexed with ''p''-coumaric acid.  Active His is shown in black.]]
*[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=169745 Tyrosine Ammonia Lyase] (TAL) - Begins the resveratrol biosynthetic pathway by catalyzing the the conversion of L-tyrosine to ammonia and ''p''-coumaric acid.  TAL also has activity as Phenylalanine Ammonia Lyase (PAL) via conversion of L-phenylalanine to ammonia and ''trans''-cinnamic acid.  The [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=36575&Template=fungiYeast ''Rhodotorula glutinis''] TAL was selected specifically because of its relatively high TAL/PAL activity ratio, and its successful expression in ''Saccharomyces cerevisiae'' and ''Escherichia coli''.
*[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=169745 Tyrosine Ammonia Lyase] (TAL) - Begins the resveratrol biosynthetic pathway by catalyzing the the conversion of L-tyrosine to ammonia and ''p''-coumaric acid.  TAL also has activity as Phenylalanine Ammonia Lyase (PAL) via conversion of L-phenylalanine to ammonia and ''trans''-cinnamic acid.  The [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=36575&Template=fungiYeast ''Rhodotorula glutinis''] TAL was selected specifically because of its relatively high TAL/PAL activity ratio, and its successful expression in ''Saccharomyces cerevisiae'' and ''Escherichia coli''.
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[[Image:TAL catalysis.png|left|600px]]
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Revision as of 08:13, 29 October 2008


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SUMMARY ::: BACKGROUND ::: STRATEGY ::: CONSTRUCTS ::: RESULTS ::: ONGOING WORK ::: OUR TEAM ::: NOTEBOOK ::: GALLERY

Contents

Metabolic Parts

TAL monomer complexed with p-coumaric acid. Active His is shown in black.
  • Tyrosine Ammonia Lyase (TAL) - Begins the resveratrol biosynthetic pathway by catalyzing the the conversion of L-tyrosine to ammonia and p-coumaric acid. TAL also has activity as Phenylalanine Ammonia Lyase (PAL) via conversion of L-phenylalanine to ammonia and trans-cinnamic acid. The Rhodotorula glutinis TAL was selected specifically because of its relatively high TAL/PAL activity ratio, and its successful expression in Saccharomyces cerevisiae and Escherichia coli.
TAL catalysis.png













Circuit Design

Cellular Chassis

Fermentation Apparatus

SUMMARY ::: BACKGROUND ::: STRATEGY ::: CONSTRUCTS ::: RESULTS ::: ONGOING WORK ::: OUR TEAM ::: NOTEBOOK ::: GALLERY